treadmill 运动对压力性尿失禁大鼠漏点压和脑神经元激活的影响。
Effect of Treadmill Exercise on Leak-point pressure and Neuronal Activation in Brain of Rats with Stress Urinary Incontinence.
机构信息
Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea.
出版信息
Int Neurourol J. 2010 Oct;14(3):141-8. doi: 10.5213/inj.2010.14.3.141. Epub 2010 Oct 31.
PURPOSE
Stress urinary incontinence (SUI) commonly occurs in women, and it causes enormous impact on quality of life. Surgery, drugs, and exercise have been recommended for the treatment of this disease. Among these exercise is also known to be effective for relieving thesymptoms of SUI, however, the efficacy and underlying mechanisms of exercise on SUI are poorly understood. In the present study, we investigated the effect of treadmill exercise on abdominal leak-point pressure and neuronal activity in the medial preoptic nucleus (MPA), ventrolateral periaqueductal gray (vlPAG), and pontine micturition center (PMC) following urethrolysis in rats.
MATERIALS AND METHODS
Adult female Sprague-Dawley rats, weighing 250±10 g (9 weeks old), were used in this study. After having undergone transabdominal urethrolysis to induce SUI, the rats were divided into three groups (n=6 in each group): a sham operation group, an SUI-induced group, and an SUI-induced and treadmill exercise group. The rats in the exercise group performed treadmill running for 30 min once a day starting 2 weeks after the induction of SUI and continuing for 4 weeks after surgery. For this study, determination of abdominal leak point pressure and immunohistochemistry for c-Fos in the brain were performed.
RESULTS
Induction of transabdominal urethrolysis significantly reduced the abdominal leak point pressure, thereby contributing to the induction of SUI. In contrast, abdominal leak point pressure was significantly improved by treadmill exercise. The expression of c-Fosin the MPA, vlPAG, and PMC, the brain areas relating to micturition, was enhanced by the induction of SUI, whereas treadmill exercise significantly suppressed SUI-induced c-Fos expression, suggesting that neuronal activation in the micturition centers was suppressed by treadmill exercise.
CONCLUSION
The present results suggest that treadmill exercise may be an effective therapeutic modality for ameliorating the symptoms of SUI.
目的
压力性尿失禁（SUI）常见于女性，对生活质量有很大影响。手术、药物和运动已被推荐用于治疗这种疾病。其中，运动也被认为对缓解 SUI 症状有效，然而，运动对 SUI 的疗效和潜在机制尚不清楚。在本研究中，我们研究了跑台运动对尿道切开术后大鼠腹压漏点压和中脑导水管周围灰质（MPA）、腹外侧导水管周围灰质（vlPAG）和桥脑排尿中枢（PMC）神经元活性的影响。
材料和方法
成年雌性 Sprague-Dawley 大鼠，体重 250±10 g（9 周龄），用于本研究。在经腹尿道切开术后诱导 SUI 后，大鼠分为三组（每组 6 只）：假手术组、SUI 诱导组和 SUI 诱导和跑台运动组。运动组大鼠在 SUI 诱导后 2 周开始每天进行 30 min 跑台运动，持续 4 周。在本研究中，进行了腹压漏点压测定和脑内 c-Fos 免疫组化检测。
结果
经腹尿道切开术诱导显著降低了腹压漏点压，从而导致 SUI 的发生。相反，跑台运动显著改善了腹压漏点压。MPA、vlPAG 和 PMC 中的 c-Fos 表达在脑与排尿相关的区域增强，而跑台运动显著抑制了 SUI 诱导的 c-Fos 表达，提示跑台运动抑制了排尿中枢的神经元激活。
结论
本研究结果提示跑台运动可能是改善 SUI 症状的有效治疗方法。
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