Qian Ling-bo, Wang Hui-ping, Huang He, Ma Xia, Xia Qiang
Department of Physiology, Zhejiang University School of Medicine, Hangzhou 310058, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2007 May;23(2):204-9.
To investigate the changes in aortic functions in streptozotocin(STZ)-induced diabetic rats and the effect of interleukin-2 (IL-2) on them, we observed the vasorelaxation of aorta to acetylcholine(ACh) and sodium nitroprusside(SNP).
Male Sprague-Dawley rats were randomly divided into a normal control group, an IL-2 control group, a diabetic group, and diabetic groups administered with a low dose (5 x 10(3) U x kg(-1) x d(-1), s.c.) or a high dose of IL-2 (5 x 10(4) U x kg(-1) x d(-1), s.c.) for five weeks. Aortic rings were isolated for use in vitro isometric force recording studies, and endothelium-dependent relaxation induced by ACh and endothelium-independent relaxation induced by SNP were measured. The serum nitric oxide (NO) levels and the activities of serum superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) were measured.
ACh caused a dose-dependent relaxation that was weakened in the diabetic group. The IL-2 treated groups were less weakened. However, the endothelium-independent relaxation induced by SNP was not significantly different in aortae of all groups. The serum NO levels were significantly increased in diabetic rats treated with IL-2 when compared with diabetic group, but the serum SOD and GSH-PX activities were not improved in diabetic group with IL-2.
IL-2 can improve the aortic endothelium-dependent relaxation in diabetic rats, which involved the improvement of endothelial function in aorta, other than the alteration of anti-oxidative capacity.
为研究链脲佐菌素（STZ）诱导的糖尿病大鼠主动脉功能的变化以及白细胞介素-2（IL-2）对其的影响，我们观察了主动脉对乙酰胆碱（ACh）和硝普钠（SNP）的血管舒张作用。
将雄性Sprague-Dawley大鼠随机分为正常对照组、IL-2对照组、糖尿病组，以及给予低剂量（5×10³ U·kg⁻¹·d⁻¹，皮下注射）或高剂量IL-2（5×10⁴ U·kg⁻¹·d⁻¹，皮下注射）的糖尿病组，持续五周。分离主动脉环用于体外等长力记录研究，测量ACh诱导的内皮依赖性舒张和SNP诱导的非内皮依赖性舒张。检测血清一氧化氮（NO）水平以及血清超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-PX）的活性。
ACh引起剂量依赖性舒张，在糖尿病组中减弱。IL-2治疗组减弱程度较小。然而，SNP诱导的非内皮依赖性舒张在所有组的主动脉中无显著差异。与糖尿病组相比，IL-2治疗的糖尿病大鼠血清NO水平显著升高，但IL-2治疗的糖尿病组血清SOD和GSH-PX活性未改善。
IL-2可改善糖尿病大鼠主动脉的内皮依赖性舒张，这涉及主动脉内皮功能的改善，而非抗氧化能力的改变。
