Yang Xiao-man, Shan Xiao-xiao, Chen Long, Cao Peng, Zhang Shuang-quan
College of Life Sciences, Nanjing Normal University, Jiangsu Engineering Research Center for Bio-medical Function Materials, Nanjing 210097, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2007 Nov;23(4):478-81.
To investigate effects of hsBAFF synthesized in Escherichia coli on spleen B lymphocyte immune response and its intracellular free Ca2+ ([Ca2]i]) signaling in mice.
Twenty ICR mice, half males-half females, were chosen and randomly divided into a normal control group (n=10) and a hsBAFF treatment group (n-10). The mice in hsBAFF treatment group were given abdominal cavity injection of hsBAFF solution which was diluted with phosphate buffered saline (PBS) at dosage of 0.1 mg/kg body weight once each day for over eight days. The mice in control group were received abdominal injection of PBS at the same dose and frequency. Spleen B lymphocyte proliferation and its immune response to LPS stimulation in mice were evaluated using an MTT assay, and change of spleen B lymphocyte [Ca2+]i was assayed under a laser scanning confocal microscope.
B lymphocyte proliferation and its immune response to LPS stimulation were significantly higher in hsBAFF-treated mice than in control mice (P < 0.05). The B lymphocyte [Ca2+]i fluorescence intensity in hsBAFF-treated mice maintained at a relatively high level fluctuation, and its average intensity was significantly higher to that of control mice (P < 0.01), but change rate of the intensity was lower compared to that of control group.
hsBAFF synthesized in Escherichia coli can enhance immune function in the body by increasing B lymphocyte proliferation and its immune response. hsBAFF-activated B lymphocyte function may be associated with increasing B lymphocytes [Ca2+]i.
研究在大肠杆菌中合成的人可溶性B细胞活化因子（hsBAFF）对小鼠脾脏B淋巴细胞免疫反应及其细胞内游离钙离子（[Ca2+]i）信号的影响。
选取20只ICR小鼠，雌雄各半，随机分为正常对照组（n=10）和hsBAFF治疗组（n=10）。hsBAFF治疗组小鼠腹腔注射用磷酸盐缓冲液（PBS）稀释的hsBAFF溶液，剂量为0.1mg/kg体重，每天1次，连续8天以上。对照组小鼠腹腔注射相同剂量和频率的PBS。采用MTT法评估小鼠脾脏B淋巴细胞增殖及其对LPS刺激的免疫反应，并在激光扫描共聚焦显微镜下检测脾脏B淋巴细胞[Ca2+]i的变化。
hsBAFF治疗组小鼠B淋巴细胞增殖及其对LPS刺激的免疫反应明显高于对照组小鼠（P<0.05）。hsBAFF治疗组小鼠B淋巴细胞[Ca2+]i荧光强度维持在较高水平波动，其平均强度明显高于对照组小鼠（P<0.01），但强度变化率低于对照组。
在大肠杆菌中合成的hsBAFF可通过增加B淋巴细胞增殖及其免疫反应来增强机体免疫功能。hsBAFF激活的B淋巴细胞功能可能与增加B淋巴细胞[Ca2+]i有关。
