[非洲爪蟾卵母细胞中人血清素转运体（hSERT）异源表达模型的建立]
[Establishment of heterologous expression model of hSERT in Xenopus laevis oocytes].
作者信息
Wang Yi-Ying, Jin Zhu, Li Ci-Zhen, Liu Yuan-Mou
机构信息
Department of Physiology, Shanghai Second Medical University, Shanghai 200025, China.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2005 Nov;21(4):444-8.
AIM
To determine the feasibility of establishing the heterologous expression model of human- serotonin transporter(hSERT or 5-HTT).
METHODS
cRNA of SERT was transcribed from cDNA, which was cloned in the pOTV vector. Each oocyte of mature xenopus laevis was injected with transcribed cRNA in vivo and incubated at room temperature for 4-9 days. Recording the current induced by 5-HT with voltage clamp technique tested the function of the expressed 5-HT transporter.
RESULTS
The transporter current could be observed in Ringer's solution containing 5-HT, and the 5-HT induced current were concentration-dependent. Norepinephrine and dopamine could not induce the transporter current while the 5-HT induced current could be specifically inhibited by 5-HTT blocker, desipramine.
CONCLUSION
The results demonstrate that the heterologous expression product in xenopus laevis oocytes is human 5-HT transporter.
目的
确定建立人血清素转运体（hSERT 或 5-HTT）异源表达模型的可行性。
方法
SERT 的 cRNA 由克隆于 pOTV 载体的 cDNA 转录而来。将转录的 cRNA 体内注射到每枚成熟非洲爪蟾卵母细胞中，并在室温下孵育 4 - 9 天。用电压钳技术记录 5 - HT 诱导的电流，以测试所表达的 5 - HT 转运体的功能。
结果
在含有 5 - HT 的林格氏液中可观察到转运体电流，且 5 - HT 诱导的电流呈浓度依赖性。去甲肾上腺素和多巴胺不能诱导转运体电流，而 5 - HT 诱导的电流可被 5 - HTT 阻断剂地昔帕明特异性抑制。
结论
结果表明非洲爪蟾卵母细胞中的异源表达产物为人 5 - HT 转运体。
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