Zuo Hong-Yan, Wu Ming-Quan, Cui Bo, She Xiao-Jun
Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2005 Nov;21(4):462-5.
To investigate the mechanism of protection by sound conditioning from acoustic trauma.
Sound conditioning experimental model of animals was established. The expression of CaM, HSP70 and F-actin in hair cells were examined with the method of immunohistochemistry. Free calcium concentration in hair cells was observed by LSCM at the same time. Quantitative investigation was devised to assess the changes of F-actin, CaM, HSP70 and intracellular calcium concentration in hair cells.
The expression of CaM, HSP70 and F-actin all showed an increased trend after noise exposure. HSP70 and F-actin expressed significantly more in group CH than that expressed in group H. Compared with group H, the expression of CaM showed an increased trend in group CH. Elevation of intracellular calcium concentration could be resulted from noise exposure. The calcium concentration in group H was significantly higher than that in group C and group CH.
A suitable sound conditioning can make the auditory system of guinea pig more resistant to noise trauma. The strengthened cytoskeleton system and the intracellular calcium homeostasis play a critical role in the protective mechanism of sound conditioning.
探讨声音预处理对声损伤的保护机制。
建立动物声音预处理实验模型。采用免疫组织化学方法检测毛细胞中钙调蛋白（CaM）、热休克蛋白70（HSP70）和F-肌动蛋白的表达。同时用激光扫描共聚焦显微镜观察毛细胞内游离钙浓度。设计定量研究以评估毛细胞中F-肌动蛋白、CaM、HSP70和细胞内钙浓度的变化。
噪声暴露后CaM、HSP70和F-肌动蛋白的表达均呈上升趋势。HSP70和F-肌动蛋白在CH组中的表达明显高于H组。与H组相比，CH组中CaM的表达呈上升趋势。噪声暴露可导致细胞内钙浓度升高。H组的钙浓度明显高于C组和CH组。
适当的声音预处理可使豚鼠听觉系统对噪声损伤更具抵抗力。强化的细胞骨架系统和细胞内钙稳态在声音预处理的保护机制中起关键作用。
