Bai Y, Piao X H, Luo Q L, Mao B Z
Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing.
Chin Med J (Engl). 1990 May;103(5):385-90.
A CD10 monoclonal antibody 55 (McAb55) was intended for purging residual common acute lymphoblastic leukemia antigen (CALLA) positive leukemic cells from autotransplants of common acute lymphoblastic leukemia (C-ALL) patients. It was found that after two rounds of McAb55 and complement treatment, 4-5 logs of CALLA+ cells were removed from bone marrow detected by clonogenic assay. The standardization of separation, purgation and preservation of bone marrow for C-ALL patients' autotransplants was then set as follows: Following the carboxymethyl starch sedimentation and Ficoll-Hypaque gradient separation, the isolated mononuclear cells (MNCs) were treated with McAb55 and complement twice and kept in room temperature for 48-72 hours prior to infusion. This procedure resulted in the removal of more than 99% of CALLA+ cells, recovery of 10-30% MNCs, and leaving the hematopoiesis stem cells intact. After the intensive cytoreductive therapy, 4 patients with C-ALL received the purged autotransplants giving timely recovery of the hematopoietic function. The patients were all remaining in remission status for more than 40-250 days so far.
一种CD10单克隆抗体55（McAb55）旨在清除普通急性淋巴细胞白血病（C-ALL）患者自体移植中的残留普通急性淋巴细胞白血病抗原（CALLA）阳性白血病细胞。发现经过两轮McAb55和补体处理后，通过克隆形成试验检测到骨髓中4-5个对数的CALLA+细胞被清除。然后将C-ALL患者自体移植骨髓的分离、净化和保存标准化如下：在羧甲基淀粉沉降和Ficoll-Hypaque梯度分离后，将分离出的单个核细胞（MNCs）用McAb55和补体处理两次，并在输注前于室温下保存48-72小时。该程序导致超过99%的CALLA+细胞被清除，MNCs回收率为10-30%，造血干细胞保持完整。在强化细胞减灭治疗后，4例C-ALL患者接受了净化后的自体移植，造血功能及时恢复。到目前为止，这些患者均保持缓解状态超过40-250天。
