Pinto Shelon Cristina Souza, Batitucci Roberta Grasseli, Pinheiro Michele Carolina, Zandim Daniela Leal, Spin-Neto Rubens, Sampaio José Eduardo Cezar
Department of Oral Diagnosis and Surgery, Araraquara Dental School, São Paulo State University, Araraquara, SP, Brazil.
Braz Dent J. 2010;21(5):390-5. doi: 10.1590/s0103-64402010000500002.
This study quantified alterations in root dentin permeability after exposure to different acid beverages. Twenty-five third molars were sectioned below the cementoenamel junction, the root segment was collected, and the pulp tissue was removed. The root segments were connected to a hydraulic pressure apparatus to measure the permeability of root dentin after the following sequential steps, with 5 specimens in each: 1) phosphoric acid etching for 30 s (maximum permeability), 2) root planning to create new smear layer, 3) exposure to different acid substances for 5 min (orange, cola drink, vinegar, white wine, lemon juice), 4) toothbrushing with sonic toothbrush for 3 min, 5) toothbrushing with sonic toothbrush plus dentifrice for 3 min. Considering step I as 100%, the data were converted into percentage and each specimen was its own control. Data were analyzed statistically by Kruskal-Wallis and Dunn's post test at 5% significance level. All acidic substances increased dentin permeability significantly after scraping (p< 0.05). Toothbrushing after exposure to acid substances decreased dentin permeability and the association with dentifrice accentuated the decrease (p< 0.05), except for the specimens treated with cola drink. Thus, it may be concluded that all tested acid fruit juices increased dentin permeability, and toothbrushing with or without dentifrice can decrease root dentin permeability after dentin exposure to acid diet.
本研究对暴露于不同酸性饮料后牙根牙本质渗透性的变化进行了量化。将25颗第三磨牙在牙骨质釉质界下方进行切片，收集牙根部分，并去除牙髓组织。将牙根部分连接到液压装置上，按以下顺序步骤测量牙根牙本质的渗透性，每组5个标本：1）磷酸酸蚀30秒（最大渗透性）；2）进行根面平整以形成新的玷污层；3）暴露于不同酸性物质5分钟（橙汁、可乐饮料、醋、白葡萄酒、柠檬汁）；4）用声波牙刷刷牙3分钟；5）用声波牙刷加牙膏刷牙3分钟。将步骤I视为100%，数据转换为百分比，每个标本作为自身对照。数据采用Kruskal-Wallis检验和Dunn事后检验进行统计学分析，显著性水平为5%。刮除后，所有酸性物质均显著增加牙本质渗透性（p<0.05）。暴露于酸性物质后刷牙可降低牙本质渗透性，且与牙膏一起使用会加剧这种降低（p<0.05），可乐饮料处理的标本除外。因此，可以得出结论，所有测试的酸性果汁都会增加牙本质渗透性，并且在牙本质暴露于酸性饮食后，使用或不使用牙膏刷牙均可降低牙根牙本质渗透性。
