Anhalt F A, Azevedo J L, Sugayama R L, Specht A, Barros N M
Centro de Ciências Agrárias e Biológicas, Instituto de Biotecnologia, Universidade de Caxias do Sul, RS, Brazil.
Braz J Biol. 2010 Nov;70(4):931-6. doi: 10.1590/s1519-69842010000500003.
Several insects are harmful to apples grown in Brazil, especially the leafroller Bonagota salubricola (Lepidoptera, Tortricidae), usually controlled with chemical insecticides. The purpose of the present study was to investigate the use of Metarhizium anisopliae strains in the control of the apple leafroller, by assessing their virulence to B. salubricola larvae in bioassays with suspensions of 2 x 10⁵ to 2 x 10⁹ conidia/mL as well as their relationship with protease expression. The most effective strain underwent a compatibility test with chemical insecticides. The M. anisopliae E6 strain showed a good performance, with up to 88% mortality and a LT₅₀ of 1.66 days. The virulence was positively correlated with a higher enzymatic activity. The E6 strain was compatible with tebufenozide, evidencing its potential to control B. salubricola.
有几种昆虫对巴西种植的苹果有害，尤其是卷叶蛾Bonagota salubricola（鳞翅目，卷蛾科），通常使用化学杀虫剂进行防治。本研究的目的是通过在生物测定中评估绿僵菌菌株对Bonagota salubricola幼虫的毒力（使用浓度为2×10⁵至2×10⁹分生孢子/毫升的悬浮液）及其与蛋白酶表达的关系，来研究绿僵菌菌株在防治苹果卷叶蛾中的应用。对最有效的菌株进行了与化学杀虫剂的相容性测试。绿僵菌E6菌株表现良好，死亡率高达88%，半数致死时间为1.66天。毒力与较高的酶活性呈正相关。E6菌株与虫酰肼相容，证明了其防治Bonagota salubricola的潜力。
