大象垂体促性腺激素。

Elephant pituitary gonadotropins.

作者信息

McFarlane J R, Cabrera C M, Oosthuizen M M, Papkoff H

机构信息

Hormone Research Institute, University of California, San Francisco 94143.

出版信息

Gen Comp Endocrinol. 1990 Aug;79(2):193-200. doi: 10.1016/0016-6480(90)90104-t.

DOI:10.1016/0016-6480(90)90104-t

PMID:2118115

Abstract

We describe for the first time the purification and some properties of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) isolated from anterior pituitary tissue of the African elephant (Loxodonta africana). Methodology previously applied to equine and donkey pituitaries was used to obtain purified preparations of elephant LH and FSH in yields of 8.8 and 0.48 mg, respectively, per 10 g pituitary powder. The preparations were characterized by HPLC gel filtration and amino acid analysis, both of which showed the elephant LH and FSH to be very similar to ovine LH and FSH. The preparations were also characterized by radioimmunoassays and bioassays for LH and FSH and a radioreceptor assay for FSH. Results showed virtually no cross-contamination of hormonal activities in the elephant LH and FSH preparations. Elephant LH potencies ranged from 50 to 66% of highly purified ovine LH and elephant FSH potencies ranged from 21 to 52% of highly purified ovine FSH in the various assays employed. No evidence was found for any demonstrable intrinsic FSH activity in elephant LH. The assays employed suggest possible usage for making physiological measurements of gonadotropins in the elephant.

摘要

我们首次描述了从非洲象（Loxodonta africana）垂体前叶组织中分离出的促黄体生成素（LH）和促卵泡激素（FSH）的纯化方法及某些特性。先前应用于马和驴垂体的方法被用于获得纯化的大象LH和FSH制剂，每10 g垂体粉末的产量分别为8.8 mg和0.48 mg。通过高效液相色谱凝胶过滤和氨基酸分析对制剂进行了表征，结果均表明大象LH和FSH与绵羊LH和FSH非常相似。还通过LH和FSH的放射免疫分析、生物分析以及FSH的放射受体分析对制剂进行了表征。结果显示大象LH和FSH制剂中激素活性几乎没有交叉污染。在所用的各种分析中，大象LH的效价为高度纯化绵羊LH的50%至66%，大象FSH的效价为高度纯化绵羊FSH的21%至52%。未发现大象LH中有任何可证明的内在FSH活性。所采用的分析表明，这些方法可能用于对大象促性腺激素进行生理学测量。