Wang Jing, Cai Jing, Li Zhimin, Hu Sha, Yu Lili, Xiao Lan, Wang Zehua
Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
J Huazhong Univ Sci Technolog Med Sci. 2010 Dec;30(6):771-6. doi: 10.1007/s11596-010-0656-5. Epub 2010 Dec 22.
The expression of N-myc down-regulated gene 1 (NDRG1) has previously been reported to be involved in the proliferation, differentiation, invasion and metastasis of cancer cells, but its role in cervical cancer is still unclear. This study aimed to investigate the expression of NDRG1gene in human cervical cancer and its effect on aggressive tumor behaviors. The NDRG1 expression in cervical tissues and cells was detected by RT-PCR. Specific expression plasmid pEGFP-N1-NDRG1-GFP was used to enhance the expression of NDRG1 in human cervical cancer cell lines. The mRNA and protein level of NDRG1 was assessed by RT-PCR and Western blotting, respectively. Its effects on cell proliferation, migration, invasion, cell cycle and apoptosis were detected by MTT, transwell migration assay and flow cytometry (FCM), respectively. The results showed that the expression of NDRG1 in cervical cancer tissues and cells was significantly lower than in normal cervical tissues (P<0.001). After transfection with pEGFP-N1-NDRG1-GFP, the mRNA and protein expression of NDRG1 was up-regulated in Siha cells, which suppressed cell proliferation (P<0.001), induced cell cycle arrest (P<0.05), reduced invasion and migration of Siha cells (P<0.05), but caused no cell apoptosis. Moreover, vascular endothelial growth factor (VEGF), a tumor-induced angiogenesis factor, was markedly reduced and E-cadherin, a cell adhesion molecule, was increased in the cells transfected with pEGFP-N1-NDRG1-GFP. It was concluded that up-regulated NDRG1 may play a role in the suppression of malignant cell growth, invasion and metastasis of human cervical cancer.
先前有报道称N - myc下调基因1（NDRG1）的表达与癌细胞的增殖、分化、侵袭和转移有关，但其在宫颈癌中的作用仍不清楚。本研究旨在探讨NDRG1基因在人宫颈癌中的表达及其对肿瘤侵袭性行为的影响。通过RT - PCR检测宫颈组织和细胞中NDRG1的表达。使用特异性表达质粒pEGFP - N1 - NDRG1 - GFP增强人宫颈癌细胞系中NDRG1的表达。分别通过RT - PCR和蛋白质印迹法评估NDRG1的mRNA和蛋白质水平。分别通过MTT、transwell迁移实验和流式细胞术（FCM）检测其对细胞增殖、迁移、侵袭、细胞周期和凋亡的影响。结果显示，宫颈癌组织和细胞中NDRG1的表达明显低于正常宫颈组织（P<0.001）。用pEGFP - N1 - NDRG1 - GFP转染后，Siha细胞中NDRG1的mRNA和蛋白质表达上调，抑制了细胞增殖（P<0.001），诱导细胞周期停滞（P<0.05），减少了Siha细胞的侵袭和迁移（P<0.05），但未引起细胞凋亡。此外，在转染pEGFP - N1 - NDRG1 - GFP的细胞中，肿瘤诱导血管生成因子血管内皮生长因子（VEGF）明显降低，细胞粘附分子E - cadherin增加。得出的结论是，上调的NDRG1可能在抑制人宫颈癌恶性细胞生长、侵袭和转移中发挥作用。
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