Wang Zhen, Wang Fang, Wang Wei-Qi, Gao Qian, Wei Wan-Li, Yang Yun, Wang Guo-Ying
Department of Pathology, Kunming Medical College, 191 Renmin xilu, Kunming 650031, Yunnan Province, China.
World J Gastroenterol. 2004 Feb 15;10(4):550-4. doi: 10.3748/wjg.v10.i4.550.
To study the function of N-myc downstream-regulated gene 1 (NDRG1) in colorectal carcinogenesis and its correlation with tumor lymph node metastasis.
NDRG1 was detected at its protein level by immunohistochemistry (IHC) and image analysis (IA), and NDRG1 mRNA was detected by in situ hybridization (ISH) in formalin-fixed and paraffin-embedded sections with a total of 190 specimens including 38 normal colorectal mucosae, 31 colorectal adenomas, 45 non-metastatic colorectal carcinomas (CRCs), 38 metastatic primary CRC and subsequently regional lymph nodes respectively. At the same time, the correlations of NDRG1 with sex, age of patients and histological types of colorectal carcinomas were observed.
NDRG1 proteins were gradually increased in colorectal carcinogenesis (P<0.05 or P<0.01). There was a significant difference in the expression of NDRG1 between non-metastatic and metastatic CRCs (P<0.05), and the correlation was positive (P<0.01, r(s)=0.329). However, there was no obvious difference in the expression of NDRG1 between the primary sites of CRCs and that in the metastatic sites of corresponding regional lymph nodes, nor was there an apparent difference in sex, age, and histological types. The expression of NDRG1 mRNA was generally in concordance with that of NDRG1 protein.
NDRG1 gene may play an important role in colorectal carcinogenesis. In addition, NDRG1 may be a putative tumor metastasis promoter gene and is regarded as one of the molecular biological markers that can forecast early metastasis of CRCs. NDRG1 gene in the metastatic sites of regional lymph nodes may preserve its expression characteristics in the primary sites of CRCs to some extent. The expression of NDRG1 is not affected by sex, age and histological types. The role of NDRG1 in tumor metastatic process can be demonstrated by in vivo and in vitro.
研究N - myc下游调控基因1（NDRG1）在结直肠癌发生中的作用及其与肿瘤淋巴结转移的相关性。
采用免疫组织化学（IHC）和图像分析（IA）在蛋白质水平检测NDRG1，采用原位杂交（ISH）在福尔马林固定石蜡包埋切片中检测NDRG1 mRNA，共检测190例标本，包括38例正常结直肠黏膜、31例结直肠腺瘤、45例非转移性结直肠癌（CRC）、38例转移性原发性CRC及其相应区域淋巴结。同时，观察NDRG1与患者性别、年龄及结直肠癌组织学类型的相关性。
NDRG1蛋白在结直肠癌发生过程中逐渐升高（P<0.05或P<0.01）。非转移性和转移性CRC中NDRG1的表达存在显著差异（P<0.05），且呈正相关（P<0.01，r(s)=0.329）。然而，CRC原发部位与相应区域淋巴结转移部位的NDRG1表达无明显差异，性别、年龄及组织学类型也无明显差异。NDRG1 mRNA的表达与NDRG1蛋白的表达总体一致。
NDRG1基因可能在结直肠癌发生中起重要作用。此外，NDRG1可能是一种假定的肿瘤转移促进基因，被视为可预测CRC早期转移的分子生物学标志物之一。区域淋巴结转移部位的NDRG1基因在一定程度上可能保留其在CRC原发部位的表达特征。NDRG1的表达不受性别、年龄和组织学类型的影响。NDRG1在肿瘤转移过程中的作用可通过体内和体外实验得到证实。
