School of Life and Health Sciences, Aston University, Birmingham B4 7ET, UK.
Int J Pharm. 2011 Sep 30;417(1-2):138-50. doi: 10.1016/j.ijpharm.2010.12.021. Epub 2010 Dec 21.
A plethora of techniques for the imaging of liposomes and other bilayer vesicles are available. However, sample preparation and the technique chosen should be carefully considered in conjunction with the information required. For example, larger vesicles such as multilamellar and giant unilamellar vesicles can be viewed using light microscopy and whilst vesicle confirmation and size prior to additional physical characterisations or more detailed microscopy can be undertaken, the technique is limited in terms of resolution. To consider the options available for visualising liposome-based systems, a wide range of microscopy techniques are described and discussed here: these include light, fluorescence and confocal microscopy and various electron microscopy techniques such as transmission, cryo, freeze fracture and environmental scanning electron microscopy. Their application, advantages and disadvantages are reviewed with regard to their use in analysis of lipid vesicles.
有许多用于脂质体和其他双层囊泡成像的技术。然而，在选择技术和样本制备时，应根据所需信息进行仔细考虑。例如，较大的囊泡，如多层囊泡和巨单层囊泡，可以使用光学显微镜观察，虽然可以在进行其他物理特性分析或更详细的显微镜检查之前，对囊泡进行确认和大小进行检查，但该技术在分辨率方面受到限制。为了考虑用于可视化基于脂质体的系统的选项，本文在这里描述和讨论了各种显微镜技术：这些技术包括光、荧光和共聚焦显微镜以及各种电子显微镜技术，如透射、冷冻、冷冻断裂和环境扫描电子显微镜。它们的应用、优点和缺点，都与它们在脂质体分析中的应用有关。
