Reproductive Biology Division, Department of Obstetrics and Gynecology, McMaster University, 1200 Main Street West, Hamilton, Ontario, Canada L8N 3Z5.
Reprod Toxicol. 2011 May;31(4):418-23. doi: 10.1016/j.reprotox.2010.12.004. Epub 2010 Dec 21.
The objective of this study was to determine the effect of in utero and lactational exposure to nicotine on the male reproductive tract. Dams were randomly assigned to receive saline or nicotine bitartrate (1mg/kg-d s.c.) daily for two weeks prior to mating until weaning (postnatal day 21). Male offspring were sacrificed at 7 (peri-pubertal) and 26 (adult) weeks of age. Nicotine-exposure resulted in retention of spermatids after stage VIII, tubular vacuolation, degeneration of pachytene and round spermatids at stage VII in the testes; and lymphocyte infiltration, germ cell exfoliation, and hypospermia in epididymides, at 7 weeks of age. Nicotine-exposure had no effect on testis or epididymal morphology, daily sperm production, epididymal sperm reserve, sperm viability at 26 weeks of age, and circulating testosterone levels at either age examined. We conclude that maternal nicotine-exposure during pregnancy and lactation can induce transient structural changes in the testis and epididymis of male offspring.
本研究旨在确定宫内和哺乳期接触尼古丁对雄性生殖道的影响。妊娠前两周和哺乳期（产后第 21 天），将母鼠随机分为盐水或尼古丁酒石酸盐（1mg/kg-d sc）组，每天给药。雄性后代分别于 7 周（青春期前）和 26 周（成年）处死。尼古丁暴露导致精子细胞在第 VIII 期后滞留、小管空泡化、精母细胞在第 VII 期退化、淋巴细胞浸润、生精细胞脱落和附睾精子数量减少，这些变化均发生于 7 周龄时。26 周龄时，尼古丁暴露对睾丸和附睾的形态、精子日产量、附睾精子储备、精子活力以及两个年龄组的循环睾酮水平均无影响。我们得出结论，母体在妊娠和哺乳期接触尼古丁可导致雄性后代睾丸和附睾的短暂结构改变。
