Ostuni Mariano A, Bizouarn Tania, Baciou Laura, Houée-Levin Chantal
Laboratoire de Chimie Physique, UMR8000, Université Paris-Sud 11, Bât 350, F-91405 Orsay, France.
Radiat Prot Dosimetry. 2011 Feb;143(2-4):166-71. doi: 10.1093/rpd/ncq518. Epub 2010 Dec 23.
The nicotinamide adenine dinucleotide phosphate oxidase complex (Nox) is a major source of non-mitochondrial reactive oxygen species in cells. Nox contains both membrane (Cytb(558)) and cytosolic (p40(phox), p47(phox), p67(phox) and Rac) components. Nox has been submitted to a combination of oxygen free radicals produced by irradiation and to hydrogen peroxide. Irradiation of a single component with high doses led to partial inactivation; however, the irradiation of the whole system during its assembly phase with lower doses (2-10 Gy) led either to activation (2.7 Gy) or to strong inactivation if irradiation took place during the first minute of the assembly. Incubation of the membrane fractions or of p67(phox) with H(2)O(2) led to fast inactivation. Catalase protected weakly p67(phox) from H(2)O(2). Conversely, incubation of the membrane fractions with catalase led to over-activation of the system.
烟酰胺腺嘌呤二核苷酸磷酸氧化酶复合物（Nox）是细胞中非线粒体活性氧的主要来源。Nox包含膜成分（细胞色素b(558)）和胞质成分（p40(phox)、p47(phox)、p67(phox)和Rac）。Nox已受到辐射产生的氧自由基和过氧化氢的作用。高剂量照射单个成分会导致部分失活；然而，在组装阶段用较低剂量（2 - 10 Gy）照射整个系统，如果在组装的第一分钟内进行照射，会导致激活（2.7 Gy）或强烈失活。膜组分或p67(phox)与H₂O₂孵育会导致快速失活。过氧化氢酶对p67(phox)免受H₂O₂的保护作用较弱。相反，膜组分与过氧化氢酶孵育会导致系统过度激活。
