基因修饰细胞输注诱导大鼠同种异体肢体免疫耐受反应。

Immunotolerance reaction for allograft-limb in rats induced by gene-modified cell transfusion.

机构信息

Department of Orthopedic Surgery, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, China.

出版信息

Panminerva Med. 2010 Dec;52(4):289-95.

Abstract

The therapeutic value of the transforming growth factor beta 1 (TGF-b) in transplantation has been reported; However, cell-mediated gene therapy using TGF-b is not applied to the organ transplantation widely. This study was to evaluate whether TGF-b-modified donor spleen cell specific transfusion in rat heterotopic allo-limb transplantation could induce tolerance tolerogenicity and prolong allograft's survival time. The Splenic T-cell in Wistar rats responsing to donor spleen cells which received TGF-b-transduced were severely impaired.The Survival time of Sprague-Dawley Allograft-limb in Wistar rats given TGF-b-modified donor spleen cells (5¥106 cells/well, administration of donor TGF-b-transduced donor spleen cells 7 days before transplantation) was extended modestly but significantly.

摘要

转化生长因子 β1（TGF-β）在移植中的治疗价值已有报道；然而，细胞介导的 TGF-β基因治疗尚未广泛应用于器官移植。本研究旨在评估 TGF-β修饰的供体脾细胞特异性输注是否能在大鼠异位同种异体肢体移植中诱导免疫耐受和延长移植物的存活时间。在接受 TGF-β转导的供体脾细胞反应的 Wistar 大鼠脾 T 细胞中，严重受损。给予 TGF-β修饰的供体脾细胞（5×106 细胞/孔，在移植前 7 天给予供体 TGF-β转导的供体脾细胞）的 Sprague-Dawley 同种异体肢体移植物在 Wistar 大鼠中的存活时间适度但显著延长。