Matsuo O, Okada K, Fukao H, Tomioka Y, Ueshima S, Watanuki M, Sakai M
Department of Physiology, Kinki University School of Medicine, Osakasayama, Japan.
Blood. 1990 Sep 1;76(5):925-9.
We evaluated the properties of recombinant staphylokinase in comparison with those of tissue-type plasminogen activator (t-PA) and streptokinase (SK). The presence of fibrin(ogen) fragment FCB-2 in the reaction mixture increased plasminogen activation by staphylokinase more than 20-fold. Such characteristics are similar to those of t-PA. On the other hand, SK was not affected by the presence of FCB-2. The thrombolytic properties of staphylokinase were studied in a system consisting of a radioactive human plasma clot (125I-fibrinogen-labeled) suspended in the circulating citrated plasma. Significant thrombolysis (50% in 3 hours) was obtained with 2 micrograms/mL of staphylokinase and 4.45 micrograms/mL t-PA, as compared with 12 micrograms/mL for SK. The relative molar potency of staphylokinase, calculated from the molecular weight, was about two times more effective than that of SK, but about half of that of t-PA. Systemic fibrinolytic activation and fibrinogen breakdown was not observed with staphylokinase or t-PA, but was observed with SK. The thrombolytic efficiency of staphylokinase, which was calculated as the ratio of the degree of thrombolysis/the degree of fibrinogenolysis, was about five times greater than that of SK, and about half of that of t-PA. These findings suggest that staphylokinase has higher specific thrombolytic properties and lesser fibrinogenolytic properties than those of SK.
我们将重组葡萄球菌激酶的特性与组织型纤溶酶原激活剂（t-PA）和链激酶（SK）的特性进行了比较。反应混合物中纤维蛋白（原）片段FCB-2的存在使葡萄球菌激酶激活纤溶酶原的能力提高了20多倍。这些特性与t-PA的特性相似。另一方面，FCB-2的存在对SK没有影响。在一个由悬浮于循环枸橼酸盐血浆中的放射性人血浆凝块（125I-纤维蛋白原标记）组成的系统中研究了葡萄球菌激酶的溶栓特性。与SK的12微克/毫升相比，2微克/毫升的葡萄球菌激酶和4.45微克/毫升的t-PA可实现显著的溶栓效果（3小时内达到50%）。根据分子量计算，葡萄球菌激酶的相对摩尔效力比SK约高两倍，但约为t-PA的一半。使用葡萄球菌激酶或t-PA未观察到全身纤溶激活和纤维蛋白原降解，但使用SK时观察到了。葡萄球菌激酶的溶栓效率（以溶栓程度/纤维蛋白原溶解程度的比值计算）约为SK的五倍，约为t-PA的一半。这些发现表明，与SK相比，葡萄球菌激酶具有更高的特异性溶栓特性和更低的纤维蛋白原溶解特性。
