交联 CD137 配体调节硫代乙醇酸盐诱导的小鼠腹腔巨噬细胞的免疫反应。
Cross-linking of CD137 ligand modulates immune responses of thioglycollate-elicited mouse peritoneal macrophages.
机构信息
Department of Pharmacology, Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, 130-701, Korea.
出版信息
Inflamm Res. 2011 May;60(5):467-73. doi: 10.1007/s00011-010-0289-y. Epub 2010 Dec 24.
OBJECTIVE
The aim of this study was to investigate effects of CD137 ligand (CD137L)-mediated reverse signaling on cellular responses in thioglycollate-elicited mouse peritoneal macrophages.
METHODS
Five-week-old male C57B6 mice were injected intraperitoneally with thioglycollate for 3 days to isolate peritoneal macrophages. We counted total cell numbers with a Cell Lab Quanta SC. CD137L expression was examined with a flow cytometer. We also measured expression of inflammatory cytokines by flow cytometry and/or real time-PCR.
RESULTS
Cross-linking of CD137L with recombinant CD137-Fc protein (rCD137-Fc) increased total numbers of thioglycollate-elicited mouse peritoneal macrophages (hIgG-Fc- vs. rCD137-Fc-treated group, p < 0.05). However, ligation reduced the increase in IL-1β and IL-6 levels. Real-time PCR analysis showed that treatment of cells with rCD137-Fc also reduced transcript levels of IL-1β, IL-6, iNOS and COX2 (hIgG-Fc- vs. rCD137-Fc-treated group, p < 0.05), as well as expression of CD137L.
CONCLUSION
Reverse signals initiated by CD137L negatively modulate certain immune functions of thioglycollate-elicited peritoneal macrophages.
目的
本研究旨在探讨 CD137 配体（CD137L）介导的反向信号对巯基乙酸盐诱导的小鼠腹腔巨噬细胞细胞反应的影响。
方法
将 5 周龄雄性 C57B6 小鼠腹腔内注射巯基乙酸盐 3 天，以分离腹腔巨噬细胞。用 Cell Lab Quanta SC 计数总细胞数。用流式细胞仪检测 CD137L 的表达。我们还通过流式细胞术和/或实时 PCR 测量炎症细胞因子的表达。
结果
用重组 CD137-Fc 蛋白（rCD137-Fc）交联 CD137L 增加了巯基乙酸盐诱导的小鼠腹腔巨噬细胞的总数（hIgG-Fc-与 rCD137-Fc 处理组相比，p < 0.05）。然而，连接减少了 IL-1β 和 IL-6 水平的增加。实时 PCR 分析表明，rCD137-Fc 处理细胞还降低了 IL-1β、IL-6、iNOS 和 COX2 的转录水平（hIgG-Fc-与 rCD137-Fc 处理组相比，p < 0.05），以及 CD137L 的表达。
结论
CD137L 启动的反向信号负调控巯基乙酸盐诱导的腹腔巨噬细胞的某些免疫功能。
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