Bai U, Lewandoski M, Dubnau E, Smith I
Department of Microbiology, New York University Medical Center, New York 10016.
J Bacteriol. 1990 Sep;172(9):5432-9. doi: 10.1128/jb.172.9.5432-5439.1990.
The initiation of sporulation in Bacillus subtilis depends on seven genes of the spo0 class. One of these, spo0F, codes for a protein of 14,000 daltons. We studied the regulation of spo0F by using spo0F-lacZ translational fusions and also measured Spo0F protein levels by immunoassays. spo0F-lacZ and Spo0F levels increased as the cells entered the stationary phase, and this effect was repressed by glucose and glutamine. Decoyinine, which lowers GTP levels and allows sporulation in the presence of normally repressing levels of glucose, induced spo0F-lacZ expression and raised Spo0F levels. The expression of spo0F-lacZ was dependent on spo0A, -0B, -0E, -0F, and -0H genes, a spo0H deletion causing the strongest effect. In most respects, the spo0F gene was regulated in a manner similar to that of spoVG. However, the presence of an abrB mutation did not relieve the dependence of spo0F gene expression on spo0A, as it does with spoVG (P. Zuber and R. Losick, J. Bacteriol. 169:2223-2230, 1987).
枯草芽孢杆菌中芽孢形成的起始依赖于spo0类的七个基因。其中之一spo0F编码一种14000道尔顿的蛋白质。我们通过使用spo0F - lacZ翻译融合体研究了spo0F的调控，并通过免疫测定法测量了Spo0F蛋白水平。当细胞进入稳定期时，spo0F - lacZ和Spo0F水平升高，并且这种效应受到葡萄糖和谷氨酰胺的抑制。脱氧助间型霉素可降低GTP水平，并能在存在通常具有抑制作用的葡萄糖水平时使芽孢形成，它可诱导spo0F - lacZ表达并提高Spo0F水平。spo0F - lacZ的表达依赖于spo0A、-0B、-0E、-0F和-0H基因，spo0H缺失产生的影响最为强烈。在大多数方面，spo0F基因的调控方式与spoVG相似。然而，abrB突变的存在并不会像spoVG那样（P. 祖伯和R. 洛西克，《细菌学杂志》169:2223 - 2230, 1987）解除spo0F基因表达对spo0A的依赖。
