表面处理和工艺参数对纤维基质吸附固定化重组酵母细胞的影响。
Effects of surface treatment and process parameters on immobilization of recombinant yeast cells by adsorption to fibrous matrices.
机构信息
Department of Chemical and Biochemical Engineering, University of Western Ontario, London, Ontario, Canada.
出版信息
Bioresour Technol. 2011 Feb;102(4):3662-72. doi: 10.1016/j.biortech.2010.11.055. Epub 2010 Nov 19.
The effects of surface properties of Saccharomyces cerevisiae strains 468/pGAC9 and 468 on adhesion to polyethyleneimine (PEI) and/glutaraldehyde (GA) pre-treated cotton (CT), polyester (PE), polyester+cotton (PECT), nylon (NL), polyurethane foam (PUF), and cellulose re-enforced polyurethane (CPU) fibers were investigated. Process parameters (circulation velocity, pH, ionic strength, media composition and surfactants) were also examined. 80%, 90%, and 35% of the cells were adsorbed onto unmodified CT, PUF, and PE, respectively. PEI-GA pre-treated CT and alkali treated PE yielded 25% and 60% cell adhesion, respectively. Adsorption rate (K(a)) ranged from 0.06 to 0.17 for CT and 0.06-0.16 for PE at varied pH. Adhesion increased by 15% in the presence of ethanol, low pH and ionic strength, and decreased by 23% in the presence of yeast extract and glucose. Shear flow and 1% Triton X-100 detached 62% and 36% nonviable cells from PE and CT, respectively, suggesting that cell immobilization in fibrous-bed bioreactors can be controlled to optimize cell density for long-term stability.
研究了酿酒酵母菌株 468/pGAC9 和 468 对经聚乙烯亚胺 (PEI) 和/或戊二醛 (GA) 预处理的棉 (CT)、聚酯 (PE)、聚酯/棉 (PECT)、尼龙 (NL)、聚氨酯泡沫 (PUF) 和纤维素增强型聚氨酯 (CPU) 纤维的表面性质的影响。还检查了工艺参数（循环速度、pH 值、离子强度、介质组成和表面活性剂）。未经修饰的 CT、PUF 和 PE 分别吸附了 80%、90%和 35%的细胞。PEI-GA 预处理的 CT 和碱性处理的 PE 的细胞粘附率分别为 25%和 60%。在不同 pH 值下，CT 的吸附速率 (K(a)) 范围为 0.06-0.17，PE 的吸附速率 (K(a)) 范围为 0.06-0.16。在乙醇、低 pH 值和离子强度存在下，粘附率增加了 15%，而在酵母提取物和葡萄糖存在下，粘附率降低了 23%。剪切流和 1% Triton X-100 从 PE 和 CT 上分别分离出 62%和 36%的非活性细胞，表明在纤维床生物反应器中可以控制细胞固定化以优化细胞密度以实现长期稳定性。
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