直接 LC-ES-MS/MS 法测定生理盐溶液中的邻苯二甲酸酯。
Direct LC-ES-MS/MS determination of phthalates in physiological saline solutions.
机构信息
Department of Analytical Chemistry, Nutrition and Bromatology, University of Santiago de Compostela, Avd de las Ciencias, Santiago de Compostela 15782, Spain.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Feb 1;879(3-4):231-5. doi: 10.1016/j.jchromb.2010.12.002. Epub 2010 Dec 10.
A method for determining a group of phthalic esters (PAEs) in physiological saline solutions has been developed. The PAEs studied were dimethyl phthalate, diethyl phthalate, butyl benzyl phthalate and dibutyl phthalate. These groups of phthalates were determined by liquid chromatography-electrospray ionization-tandem mass spectrometry, working in positive ion mode. The compounds were separated by liquid chromatography working in gradient mode with acetonitrile-ultrapure water as a mobile phase. The separation was performed starting with 5% of acetonitrile and increasing to 75% in 5 min, followed by isocratic elution for 8 min. The method was precise (with relative standard deviation (RSD) from 1.0 to 6.8%) and sensitive, with LODs of 0.05, 0.38, 0.05 and 0.82 μg L⁻¹ for DMP, DEP, BBP and DBP, respectively. The proposed analytical method has been applied to determine these compounds in different physiological saline solutions commercialized in plastic bottles.
已经开发出一种用于确定生理盐水中邻苯二甲酸酯（PAEs）的方法。研究的邻苯二甲酸酯包括邻苯二甲酸二甲酯、邻苯二甲酸二乙酯、邻苯二甲酸丁基苄基酯和邻苯二甲酸二丁酯。这些邻苯二甲酸酯通过正离子模式下的液相色谱-电喷雾串联质谱法进行测定。这些化合物通过梯度模式下的液相色谱法进行分离，以乙腈-超纯水作为流动相。分离过程从 5%的乙腈开始，在 5 分钟内增加到 75%，然后进行 8 分钟的等度洗脱。该方法具有较高的精密度（相对标准偏差（RSD）为 1.0%至 6.8%）和灵敏度，DMP、DEP、BBP 和 DBP 的检出限分别为 0.05、0.38、0.05 和 0.82 μg/L。所提出的分析方法已应用于测定不同商业化的塑料瓶装生理盐水中这些化合物的含量。
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