Sayavedra-Soto Luis A, Vajrala Neeraja, Arp Daniel J
Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon, USA.
Methods Enzymol. 2011;486:403-28. doi: 10.1016/B978-0-12-381294-0.00018-3.
The chemolithoautotroph Nitrosomonas europaea oxidizes about 25 mol of NH(3) for each mole of CO(2) that is converted to biomass using an array of heme and nonheme Fe-containing proteins. Hence mechanisms of efficient iron (Fe) uptake and homeostasis are particularly important for this Betaproteobacterium. Among nitrifiers, N.europaea has been the most studied to date. Characteristics that make N.europaea a suitable model to study Fe uptake and homeostasis are as follows: (a) its sequenced genome, (b) its capability to grow relatively well in 0.2 μM Fe in the absence of heterologous siderophores, and (c) its amenability to mutagenesis. In this chapter, we describe the methodology we use in our laboratory to dissect Fe uptake and homeostasis in the ammonia oxidizer N. europaea.
化能无机自养菌欧洲亚硝化单胞菌（Nitrosomonas europaea）每利用一系列含血红素和非血红素铁的蛋白质将1摩尔二氧化碳转化为生物质，就会氧化约25摩尔的氨（NH₃）。因此，高效铁（Fe）摄取和体内平衡机制对这种β-变形菌尤为重要。在硝化细菌中，欧洲亚硝化单胞菌是迄今为止研究最多的。使欧洲亚硝化单胞菌成为研究铁摄取和体内平衡合适模型的特征如下：（a）其已测序的基因组，（b）在没有异源铁载体的情况下，它在0.2 μM铁中相对良好生长的能力，以及（c）其对诱变的适应性。在本章中，我们描述了我们实验室用于剖析氨氧化菌欧洲亚硝化单胞菌中铁摄取和体内平衡的方法。
