Department of Environmental Engineering, Peking University, The Key Laboratory of Water and Sediment Sciences, Ministry of Education, 5 Yiheyuan Road, Haidian District, Beijing 100871, China.
J Hazard Mater. 2011 Feb 28;186(2-3):1351-4. doi: 10.1016/j.jhazmat.2010.12.013. Epub 2010 Dec 10.
The compositions of explosive wastewater generated from TNT (2,4,6-trinitrotoluene) purification stage were characterized by using UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), high performance liquid chromatograph (HPLC) and gas chromatograph/mass spectroscopy (GC/MS). The acute toxicity was evaluated by bacterium bioluminescence assay using a freshwater luminescent bacterium (Vibrio qinghaiensis sp. Nov.) and a marine luminescent bacterium (Photobacterium phosphoreum). The results showed that the wastewater's biodegradability was poor due to the high amount of chemical oxygen demand (COD). The main organic components were dinitrotoluene sulfonates (DNTS) with small amount of TNT, dinitrotoluene (DNT), mononitrotoluene (MNT) and other derivatives of nitrobenzene. It was highly toxic to luminescent bacteria P. phosphoreum and V. qinghaiensis sp. Nov. After reaction time of 15 min, the relative concentration of toxic pollutants (expressed as reciprocal of dilution ratio of wastewater) at 50% of luminescence inhibition ratio was 5.32×10(-4) for P. phosphoreu, while that was 4.34×10(-4) for V. qinghaiensis. V. qinghaiensis is more sensitive and suitable for evaluating the wastewater's acute toxicity than P. phosphoreum. After adsorption by resin, the acute toxicity can be greatly reduced, which is helpful for further treatment by biological methods.
采用紫外可见分光光度法、傅里叶变换红外光谱（FTIR）、高效液相色谱（HPLC）和气相色谱/质谱（GC/MS）对 TNT（2,4,6-三硝基甲苯）净化阶段产生的爆炸废水的成分进行了表征。采用淡水发光菌（青海弧菌）和海洋发光菌（发光杆菌）的细菌生物发光法评估了废水的急性毒性。结果表明，由于化学需氧量（COD）含量高，废水的生物降解性较差。主要有机成分是二硝基甲苯磺酸盐（DNTS），还有少量的 TNT、二硝基甲苯（DNT）、一硝基甲苯（MNT）和其他的硝基苯衍生物。它对发光菌 P. phosphoreum 和 V. qinghaiensis sp. Nov. 具有很高的毒性。在 15 分钟的反应时间后，发光抑制率为 50%时，有毒污染物的相对浓度（表示为废水稀释倍数的倒数）对于 P. phosphoreu 为 5.32×10(-4)，而对于 V. qinghaiensis 为 4.34×10(-4)。V. qinghaiensis 比 P. phosphoreum 更敏感，更适合用于评估废水的急性毒性。经树脂吸附后，急性毒性可大大降低，有助于进一步采用生物方法进行处理。
