SOE-LRed：一种简单、高效的方法，可将点突变基因定位到大肠杆菌染色体上。
SOE-LRed: A simple and time-efficient method to localize genes with point mutations onto the Escherichia coli chromosome.
机构信息
Department of Biology, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA.
出版信息
J Microbiol Methods. 2011 Mar;84(3):479-81. doi: 10.1016/j.mimet.2010.12.020. Epub 2010 Dec 24.
Abstract
We use a powerful method to replace wild-type genes on the chromosome of Escherichia coli. Using a unique form of PCR, we generate easily constructible gene fusions bearing single point mutations. Used in conjunction with homologous recombination, this method eliminates cloning procedures previously used for this purpose.
摘要
我们使用一种强大的方法来替换大肠杆菌染色体上的野生型基因。通过使用一种独特形式的 PCR，我们生成了易于构建的带有单点突变的基因融合。与同源重组结合使用，这种方法消除了以前为此目的使用的克隆程序。
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SOE-LRed: A simple and time-efficient method to localize genes with point mutations onto the Escherichia coli chromosome.SOE-LRed：一种简单、高效的方法，可将点突变基因定位到大肠杆菌染色体上。
J Microbiol Methods. 2011 Mar;84(3):479-81. doi: 10.1016/j.mimet.2010.12.020. Epub 2010 Dec 24.
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