Zhang Weiyu, Rogniaux Hélène, Huang Weiyi, Chauvin Alain, Moreau Emmanuelle
College of Animal Science and Technology, Guangxi University, 530005 Nanning, China.
Parasitol Int. 2011 Jun;60(2):206-8. doi: 10.1016/j.parint.2010.12.002. Epub 2010 Dec 23.
Buffalo fasciolosis induced by Fasciola gigantica causes important economic losses in tropical areas of Asia. Detection of prepatent infection is essential to control this disease. Classical tools such as coprology, necroscopy or ELISA based on crude extracts from F. gigantica are poorly sensitive or specific. Purified antigens could be used to increase these parameters. Western blot analysis and mass spectrometry of a fraction of F. gigantica excretory-secretory products obtained by gel filtration showed that thioredoxin peroxidase could be a potential antigen for serodiagnosis: it was recognized from the 2nd week after infection, by all buffalo experimentally or naturally infected with F. gigantica but not by healthy animals.
由巨片形吸虫引起的水牛片形吸虫病在亚洲热带地区造成了重大经济损失。检测潜伏期感染对于控制这种疾病至关重要。传统的检测方法，如粪便学、尸检或基于巨片形吸虫粗提物的酶联免疫吸附测定，其敏感性或特异性都很差。纯化抗原可用于提高这些参数。对通过凝胶过滤获得的一部分巨片形吸虫排泄-分泌产物进行的蛋白质印迹分析和质谱分析表明，硫氧还蛋白过氧化物酶可能是血清学诊断的潜在抗原：在感染后的第2周，所有经实验或自然感染巨片形吸虫的水牛都能识别该抗原，而健康动物则不能。
