Raina O K, Yadav S C, Sriveny D, Gupta S C
Division of Parasitology, Indian Veterinary Research Institute, Izatnagar 243122, India.
Acta Trop. 2006 May;98(2):145-51. doi: 10.1016/j.actatropica.2006.03.004. Epub 2006 May 2.
Fasciola gigantica cathepsin-L cysteine proteinase and recombinant cathepsin L 1-D were assessed for their potential in the immuno-diagnosis of F. gigantica infection in buffaloes. A diagnostic ELISA, based on these two antigens, was developed to detect antibodies against F. gigantica in water buffaloes. Sensitivity of the ELISA was assessed using sera from buffaloes experimentally or naturally infected with F. gigantica from F. gigantica endemic areas and its specificity by probing the sera of the host from F. gigantica non-endemic area. Our earlier studies under experimental setting showed 100% sensitivity of cathepsin-L ELISA in the diagnosis of fasciolosis in buffaloes, with the earliest detection of infection at 4 weeks post-infection. However, under field situation of natural F. gigantica infection, this sensitivity declined to 97.1% but specificity of the test remained 100%. Cross-reactivity of the antigen was checked with Schistosoma indicum, S. spindale, Paramphistomum epiclitum, Gastrothylax spp., Gigantocotyle explanatum, hydatid and Strongyloides papilossus in the bubaline host, naturally infected with these helminths. F. gigantica cathepsin-L and the recombinant cathepsin L-1D does not cross-react with these helminth parasites in natural mono or mixed infection of the host. The present ELISA contributes a relatively sensitive and reliable tool for the early serodiagnosis of bubaline fasciolosis.
对巨片形吸虫组织蛋白酶-L半胱氨酸蛋白酶和重组组织蛋白酶L 1-D在水牛巨片形吸虫感染免疫诊断中的潜力进行了评估。基于这两种抗原开发了一种诊断性酶联免疫吸附测定（ELISA），用于检测水牛体内针对巨片形吸虫的抗体。使用来自巨片形吸虫流行区经实验感染或自然感染巨片形吸虫的水牛血清评估ELISA的敏感性，并通过检测来自非巨片形吸虫流行区宿主的血清来评估其特异性。我们早期在实验环境下的研究表明，组织蛋白酶-L ELISA在诊断水牛片形吸虫病时敏感性为100%，感染后最早在4周可检测到感染。然而，在自然感染巨片形吸虫的野外情况下，这种敏感性降至97.1%，但检测的特异性仍为100%。在自然感染这些蠕虫的水牛宿主中，检查了该抗原与印度血吸虫、纺锤血吸虫、后睾吸虫、腹袋吸虫属、巨盘吸虫、包虫和乳头类圆线虫的交叉反应性。在宿主自然单一或混合感染中，巨片形吸虫组织蛋白酶-L和重组组织蛋白酶L-1D与这些蠕虫寄生虫无交叉反应。目前的ELISA为水牛片形吸虫病的早期血清诊断提供了一种相对敏感和可靠的工具。
