抗 CD25 单克隆抗体治疗抑制骨肉瘤模型中的肿瘤转移。
Suppression of tumour metastasis in a murine osteosarcoma model with anti-CD25 monoclonal antibody treatment.
机构信息
Department of Orthopaedic Surgery, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
出版信息
Anticancer Res. 2010 Dec;30(12):5019-22.
BACKGROUND
Mouse regulatory T cells (Treg) may be deleted by intraperitoneal injection of anti-CD25 monoclonal antibody (mAb) (PC61). When Treg populations are thus suppressed, the immune system attacks tumours in autoimmune reactions.
MATERIALS AND METHODS
An osteosarcoma (LM8) was transplanted subcutaneously into C3H/He mice. Serial injections of PC61 were conducted from seven days before (pre-PC61 group) or from two days thereafter (post-PC61 group) and tumour growth and metastasis were examined four weeks later. A control group received PBS injections.
RESULTS
Subcutaneous tumours were reduced in size and the numbers of lung and liver metastatic colonies were significantly decreased in both pre- and post-PC61 groups compared to the control group.
CONCLUSION
Tumour suppression was effective even when injection of PC61 was performed two days after LM8 transplantation. These results indicate that such treatments might be suitable to be applied in the clinic after surgical operations.
背景
腹腔注射抗 CD25 单克隆抗体（mAb）（PC61）可能会删除小鼠调节性 T 细胞（Treg）。当 Treg 群体受到抑制时，免疫系统会在自身免疫反应中攻击肿瘤。
材料和方法
将骨肉瘤（LM8）皮下移植到 C3H/He 小鼠中。从移植前七天（预 PC61 组）或此后两天（后 PC61 组）开始进行 PC61 的连续注射，并在四周后检查肿瘤生长和转移情况。对照组接受 PBS 注射。
结果
与对照组相比，预 PC61 组和后 PC61 组的皮下肿瘤体积均减小，肺和肝转移灶的数量也明显减少。
结论
即使在 LM8 移植后两天进行 PC61 注射，肿瘤抑制仍然有效。这些结果表明，此类治疗方法在手术后的临床应用中可能是合适的。
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