McLauchlin J, Greenwood M H, Pini P N
Division of Microbiological Reagents and Quality Control, Central Public Health Laboratory, London, U.K.
Int J Food Microbiol. 1990 May;10(3-4):255-62. doi: 10.1016/0168-1605(90)90073-e.
A case of listeriosis was associated with the consumption of a soft cheese produced in England. Goats cheese and other products from the same food manufacturer were examined for the presence of Listeria over the following 11 months. Listeria monocytogenes was isolated from 16 of 25 cheese samples on retail sale, 12 of 24 cheese samples obtained directly from the factory, and from shelving within the plant. Phage-typing of 68 isolates of L. monocytogenes from cheese samples and the factory showed that 66 (97%) were indistinguishable from the strain isolated from the patient's cerebrospinal fluid and stool. L. monocytogenes was not isolated from seven goats milk or two yoghurt samples. Listeria innocua was isolated from 10 cheese samples, two of which contained no other species of Listeria. Levels of L. monocytogenes shortly after production were low (less than 10/g), but were higher (10(5)-10(7) cfu/g) in six of the 16 cheese samples obtained from retail outlets. Multiplication of L. monocytogenes was demonstrated in cheeses contaminated at the factory and held at 4 degrees C in the laboratory.
一例李斯特菌病与食用英国生产的软奶酪有关。在接下来的11个月里，对同一家食品制造商生产的山羊奶酪及其他产品进行了李斯特菌检测。从零售的25份奶酪样本中的16份、直接从工厂获取的24份奶酪样本以及工厂内的货架上分离出了单核细胞增生李斯特菌。对从奶酪样本和工厂分离出的68株单核细胞增生李斯特菌进行噬菌体分型，结果显示其中66株（97%）与从患者脑脊液和粪便中分离出的菌株无法区分。在7份山羊奶样本或2份酸奶样本中未分离出单核细胞增生李斯特菌。从10份奶酪样本中分离出了无害李斯特菌，其中两份不含其他李斯特菌种。生产后不久，单核细胞增生李斯特菌的含量较低（低于10/g），但从零售店获取的16份奶酪样本中的6份含量较高（10⁵ - 10⁷ cfu/g）。在工厂被污染并在实验室4摄氏度保存的奶酪中证实了单核细胞增生李斯特菌的繁殖。
