Institute for Genome Sciences and Policy, Duke University Medical Center, Durham, North Carolina, United States of America.
PLoS One. 2010 Dec 20;5(12):e15271. doi: 10.1371/journal.pone.0015271.
A strand-specific transcriptome sequencing strategy, directional ligation sequencing or DeLi-seq, was employed to profile antisense transcriptome of Schizosaccharomyces pombe. Under both normal and heat shock conditions, we found that polyadenylated antisense transcripts are broadly expressed while distinct expression patterns were observed for protein-coding and non-coding loci. Dominant antisense expression is enriched in protein-coding genes involved in meiosis or stress response pathways. Detailed analyses further suggest that antisense transcripts are independently regulated with respect to their sense transcripts, and diverse mechanisms might be potentially involved in the biogenesis and degradation of antisense RNAs. Taken together, antisense transcription may have profound impacts on global gene regulation in S. pombe.
采用链特异性转录组测序策略（定向连接测序或 DeLi-seq）来分析酿酒酵母的反义转录组。在正常和热休克条件下，我们发现多聚腺苷酸化的反义转录本广泛表达，而蛋白质编码和非编码基因座表现出不同的表达模式。反义表达优势富集在减数分裂或应激反应途径中涉及的蛋白质编码基因。详细分析进一步表明，反义转录物与其有义转录物独立调控，并且可能涉及多种机制参与反义 RNA 的生物发生和降解。总之，反义转录可能对 S. pombe 的全局基因调控产生深远影响。
