The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
Nat Methods. 2010 Feb;7(2):130-2. doi: 10.1038/nmeth.1417. Epub 2010 Jan 17.
We report an alternative approach to transcriptome sequencing for the Illumina Genome Analyzer, in which the reverse transcription reaction takes place on the flowcell. No amplification is performed during the library preparation, so PCR biases and duplicates are avoided, and because the template is poly(A)(+) RNA rather than cDNA, the resulting sequences are necessarily strand-specific. The method is compatible with paired- or single-end sequencing.
我们报告了一种替代的 Illumina 基因组分析仪转录组测序方法，其中逆转录反应在流动槽上进行。在文库制备过程中不进行扩增，因此避免了 PCR 偏倚和重复，并且由于模板是 poly(A)(+) RNA 而不是 cDNA，因此得到的序列必然是链特异性的。该方法与配对或单端测序兼容。
