Vahedi Fatemeh, Jaafari Mohammad Reza, Mahmoudi Mahmoud
Razi Vaccine and Serum Research Institute, Mashhad, Iran.
Iran J Immunol. 2010 Dec;7(4):210-6.
DNA vaccines are third generation vaccines which have made promises to combat infectious diseases. Cationic liposomes are used as effective delivery systems for DNA vaccines to generate stronger immunity.
Encapsulation of pcDNA3.1+PA plasmid, encoding protective antigen (PA) of Bacillus anthracis (B. anthracis) into cationic liposomes, and evaluation of its effect on specific humoral specific immunity against PA were aimed.
The liposomes containing pcDNA3.1+PA plasmids were prepared with phosphatidylcholine (PC), dioleoyl phosphatidylethanolamine (DOPE) and 1, 2-dioleoyl-3-trimethylammonium-propane (DOTAP) using dehydration-rehydration method. BALB/c mice were immunized by intramuscular (IM) injection to investigate the immunogenicity of the formulations. The resulting specific antibodies against PA, total IgG, IgG1, IgG2a and IgG2b isotypes, were evaluated by enzyme linked immunosorbent assay (ELISA) method.
A higher concentration of specific IgG against PA was found in sera of a group immunized with the encapsulated plasmid compared with the naked plasmid alone. This difference was significant for IgG1 isotype.
DNA疫苗是第三代疫苗，有望用于对抗传染病。阳离子脂质体用作DNA疫苗的有效递送系统，以产生更强的免疫力。
将编码炭疽芽孢杆菌保护性抗原（PA）的pcDNA3.1+PA质粒封装到阳离子脂质体中，并评估其对针对PA的特异性体液免疫的影响。
采用脱水再水化法，用磷脂酰胆碱（PC）、二油酰磷脂酰乙醇胺（DOPE）和1,2-二油酰-3-三甲基铵丙烷（DOTAP）制备含pcDNA3.1+PA质粒的脂质体。通过肌肉注射免疫BALB/c小鼠，以研究制剂的免疫原性。采用酶联免疫吸附测定（ELISA）法评估所得针对PA的特异性抗体、总IgG、IgG1、IgG2a和IgG2b亚型。
与单独的裸质粒相比，在用封装质粒免疫的组的血清中发现了更高浓度的针对PA的特异性IgG。这种差异对于IgG1亚型具有显著性。
