[脱氢紫堇碱对豚鼠心肌细胞缺氧时细胞内游离钙浓度的影响]

[The influence of dehydrocorydaline on intracellular free calcium concentration during hypoxia in myocardial cell of guinea-pigs].

作者信息

Zhao Xin, Tang Hao, Wang Ya-Jie, Yu Xin, Liu Ying, Zhang Jie, Qin Jia, Guo Shan-Fen

机构信息

Department of Physiology, College of Basic Medical Sciences, China Medical University, Shenyang 110001, China.

出版信息

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2003 Aug;19(3):222-5.

PMID:21189579

Abstract

AIM

To study the effect of Dehydrocorydaline and Verapamil (Ver) on intracellular free calcium concentration of myocardial cell ([Ca2+]i) under hypoxic condition.

METHODS

We adopted guinea-pig heart Langendorff instillation. The myocardial cells were isolated by collagenase (Type I, sigma)and marked by fluorescence ratio imaging. The suspension of myocardial cells was assigned to six groups: DHC, Ver, and control were each two. Each three groups was exposed to hypoxia and normoxia before determination of [Ca2+]i.

RESULTS

(1) In normoxia state, [Ca2+]i was 120.5-8.3 nml/L (n = 20).( 2) In hypoxia state, the increased [Ca2+]i of myocardial cells was proportional to the time (degree) of hypoxia. Correlation coefficient (r) was about 0.98. (3) Under the condition of normoxia DHC and Ver decreased [Ca2+]i. (4) DHC was obviously slow the increase of [Ca2+]i after hypoxia.

CONCLUSION

In normoxia and hypoxia, DHC decreases the increased [Ca2+]i. It can prevent intracellular calcium overload. We believe DHC may improve self-protected performance of myocardial cells.

摘要

目的

研究去氢紫堇碱和维拉帕米（Ver）对缺氧状态下心肌细胞内游离钙浓度（[Ca2+]i）的影响。

方法

采用豚鼠心脏Langendorff灌流法。用I型胶原酶（sigma）分离心肌细胞，并用荧光比率成像法进行标记。将心肌细胞悬液分为六组：去氢紫堇碱组、维拉帕米组和对照组，每组各两组。在测定[Ca2+]i之前，每组中的三组分别暴露于缺氧和常氧环境。

结果

（1）在常氧状态下，[Ca2+]i为120.5 - 8.3 nml/L（n = 20）。（2）在缺氧状态下，心肌细胞[Ca2+]i的升高与缺氧时间（程度）成正比。相关系数（r）约为0.98。（3）在常氧条件下，去氢紫堇碱和维拉帕米可降低[Ca2+]i。（4）去氢紫堇碱可明显减缓缺氧后[Ca2+]i的升高。