[糖皮质激素上调人绒毛膜11β-羟类固醇脱氢酶1型]
[Glucocorticoids up-regulate human chorion 11beta-hydroxysteroid dehydrogenase type 1].
作者信息
He Ping, Sun Gang
机构信息
Department of Physiology, Second Military Medical University, Shanghai 200433, China.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2003 Aug;19(3):291-4.
AIM
To study the co-localization of glucocorticoid receptor and 11beta-hydroxysteroid dehydrogenase 1 (11beta-HSD1) and to investigate whether glucocorticoids regulate the reductase activity and expression of 11beta-HSD1.
METHODS
Immunohistochemical staining for 11beta-HSD1 in cultured primary human chorionic trophoblasts was performed. Radiometric conversion assay and Northern blot analysis were respectively used to observe the activity and mRNA expression of 11beta-HSD1.
RESULTS
11beta-HSD1 and GR were co-expressed in the same chorionic trophoblast. Both 11beta-HSD1 reductase activity and mRNA levels were increased by dexamethasone (10(-6) mol/L, 10(-7) mol/L) in the cultured chorionic trophoblasts, and the effects were blocked by GR antagonist RU486 (10(-6) mol/L).
CONCLUSION
By binding to GR, glucocorticoids induce the expression of 11beta-HSD1 by a possible intracrine mechanism.
目的
研究糖皮质激素受体与11β-羟基类固醇脱氢酶1（11β-HSD1）的共定位，并探讨糖皮质激素是否调节11β-HSD1的还原酶活性及表达。
方法
对培养的原代人绒毛膜滋养层细胞进行11β-HSD1免疫组织化学染色。分别采用放射转化分析和Northern印迹分析观察11β-HSD1的活性及mRNA表达。
结果
11β-HSD1和GR在同一绒毛膜滋养层细胞中共表达。地塞米松（10⁻⁶mol/L、10⁻⁷mol/L）可使培养的绒毛膜滋养层细胞中11β-HSD1还原酶活性及mRNA水平升高，且这些作用被GR拮抗剂RU486（10⁻⁶mol/L）阻断。
结论
糖皮质激素通过与GR结合，可能以内分泌机制诱导11β-HSD1的表达。
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