体内定向蛋白质进化技术。
Technologies of directed protein evolution in vivo.
机构信息
Institute of Protein Research, Russian Academy of Sciences, Institutskaya St. 4, 142290, Pushchino, Russian Federation.
出版信息
Cell Mol Life Sci. 2011 Apr;68(7):1207-14. doi: 10.1007/s00018-010-0610-5. Epub 2010 Dec 29.
Abstract
Directed evolution of proteins for improved or modified functionality is an important branch of modern biotechnology. It has traditionally been performed using various in vitro methods, but more recently, methods of in vivo artificial evolution come into play. In this review, we discuss and compare prokaryotic and eukaryotic-based systems of directed protein evolution in vivo, highlighting their benefits and current limitations and focusing on the biotechnological potential of vertebrate immune cells for the generation of protein diversity by means of the immunoglobulin diversification machinery.
摘要
定向进化蛋白质以改善或修饰其功能是现代生物技术的一个重要分支。传统上，它是使用各种体外方法来完成的，但最近，体内人工进化方法开始发挥作用。在这篇综述中，我们讨论和比较了原核和真核生物体内定向蛋白质进化的系统，强调了它们的优点和当前的局限性，并重点关注脊椎动物免疫细胞通过免疫球蛋白多样化机制产生蛋白质多样性的生物技术潜力。
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