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体内定向蛋白质进化技术。

Technologies of directed protein evolution in vivo.

机构信息

Institute of Protein Research, Russian Academy of Sciences, Institutskaya St. 4, 142290, Pushchino, Russian Federation.

出版信息

Cell Mol Life Sci. 2011 Apr;68(7):1207-14. doi: 10.1007/s00018-010-0610-5. Epub 2010 Dec 29.

DOI:10.1007/s00018-010-0610-5
PMID:21190058
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11115086/
Abstract

Directed evolution of proteins for improved or modified functionality is an important branch of modern biotechnology. It has traditionally been performed using various in vitro methods, but more recently, methods of in vivo artificial evolution come into play. In this review, we discuss and compare prokaryotic and eukaryotic-based systems of directed protein evolution in vivo, highlighting their benefits and current limitations and focusing on the biotechnological potential of vertebrate immune cells for the generation of protein diversity by means of the immunoglobulin diversification machinery.

摘要

定向进化蛋白质以改善或修饰其功能是现代生物技术的一个重要分支。传统上，它是使用各种体外方法来完成的，但最近，体内人工进化方法开始发挥作用。在这篇综述中，我们讨论和比较了原核和真核生物体内定向蛋白质进化的系统，强调了它们的优点和当前的局限性，并重点关注脊椎动物免疫细胞通过免疫球蛋白多样化机制产生蛋白质多样性的生物技术潜力。

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本文引用的文献

1
PCRless library mutagenesis via oligonucleotide recombination in yeast.酵母中寡核苷酸重组的无 PCR 文库诱变。
Protein Sci. 2010 Dec;19(12):2336-46. doi: 10.1002/pro.513.
2
Random mutagenesis methods for in vitro directed enzyme evolution.体外定向酶进化的随机诱变方法。
Curr Protein Pept Sci. 2010 Feb;11(1):91-100. doi: 10.2174/138920310790274617.
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Protein engineering of microbial enzymes.微生物酶的蛋白质工程。
Curr Opin Microbiol. 2010 Jun;13(3):274-82. doi: 10.1016/j.mib.2010.01.010. Epub 2010 Feb 17.
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Directed evolution: new parts and optimized function.定向进化：新的部分和优化的功能。
Curr Opin Biotechnol. 2009 Aug;20(4):486-91. doi: 10.1016/j.copbio.2009.08.005. Epub 2009 Aug 31.
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Programming cells by multiplex genome engineering and accelerated evolution.通过多重基因组工程和加速进化对细胞进行编程。
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Conditional gene silencing of multiple genes with antisense RNAs and generation of a mutator strain of Escherichia coli.利用反义RNA对多个基因进行条件性基因沉默以及大肠杆菌突变菌株的构建
Nucleic Acids Res. 2009 Aug;37(15):e103. doi: 10.1093/nar/gkp498. Epub 2009 Jun 10.
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A cis-acting diversification activator both necessary and sufficient for AID-mediated hypermutation.一种对AID介导的高突变既必要又充分的顺式作用多样化激活因子。
PLoS Genet. 2009 Jan;5(1):e1000332. doi: 10.1371/journal.pgen.1000332. Epub 2009 Jan 9.
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Activation-induced cytidine deaminase-mediated hypermutation in the DT40 cell line.激活诱导的胞苷脱氨酶介导的DT40细胞系超突变
Philos Trans R Soc Lond B Biol Sci. 2009 Mar 12;364(1517):639-44. doi: 10.1098/rstb.2008.0202.
9
Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity.针对表达、稳定性和结合选择性对G蛋白偶联受体进行定向进化。
Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):14808-13. doi: 10.1073/pnas.0803103105. Epub 2008 Sep 23.
10
Protein design by directed evolution.通过定向进化进行蛋白质设计。
Annu Rev Biophys. 2008;37:153-73. doi: 10.1146/annurev.biophys.37.032807.125832.