Nirantar Saurabh Rajendra
Illumina Singapore Inc., Singapore 757716, Singapore.
Molecules. 2021 Sep 15;26(18):5599. doi: 10.3390/molecules26185599.
Enzymes underpin the processes required for most biotransformations. However, natural enzymes are often not optimal for biotechnological uses and must be engineered for improved activity, specificity and stability. A rich and growing variety of wet-lab methods have been developed by researchers over decades to accomplish this goal. In this review such methods and their specific attributes are examined.
酶是大多数生物转化所需过程的基础。然而，天然酶通常并非最适合生物技术应用，必须对其进行改造以提高活性、特异性和稳定性。几十年来，研究人员开发了丰富且不断增加的各种湿实验室方法来实现这一目标。在本综述中，将对这些方法及其具体特性进行研究。
