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碱菌处理废水及两种生物反应器中微生物群落的动态变化。

Wastewater treatment by alkali bacteria and dynamics of microbial communities in two bioreactors.

机构信息

College of Life Sciences, Henan Normal University, Xinxiang 453007, China.

出版信息

Bioresour Technol. 2011 Feb;102(4):3790-8. doi: 10.1016/j.biortech.2010.12.030. Epub 2010 Dec 13.

DOI:10.1016/j.biortech.2010.12.030
PMID:21190847
Abstract

In this study, an alkali bacterial consortium was obtained by enrichment cultivation and was used to treat printing and dyeing wastewater (PDW, pH 11-12). The treatment effects and dynamic changes were evaluated in a biocontact oxidation reactor (BOR) and a sequencing batch reactor (SBR). During 3 months of continuous operation, the two bioreactors had similar treatment efficiencies (polyvinyl alcohol, PVA, 74.5-81.3%; COD, 73.5-77.4%; 2.15 pH decreases). Molecular biological analysis indicated that the microbial communities underwent dramatic changes during the operation, in which the SBR was superior to the BOR in retaining the alkali bacteria at the start-up stage, however, the BOR seemed to be more advantageous when the frequently changing influents were considered. The bacterial communities in BOR and SBR were diverse and included Bacteroidetes, Firmicutes, Proteobacteria, Actinobacteria and an unidentified cluster. Among these only Paracoccus sp. was successfully isolated and confirmed to have the ability to degrade PVA.

摘要

在本研究中,通过富集培养获得了一株堿性细菌混合菌,并将其用于处理印染废水(PDW,pH 值为 11-12)。在生物接触氧化反应器(BOR)和序批式反应器(SBR)中评估了其处理效果和动态变化。在连续运行 3 个月期间,两个生物反应器的处理效率相似(聚乙烯醇,PVA,74.5-81.3%;COD,73.5-77.4%;pH 值降低 2.15)。分子生物学分析表明,在运行过程中微生物群落发生了剧烈变化,其中 SBR 在启动阶段更有利于保留堿性细菌,而当考虑到频繁变化的进水时,BOR 似乎更有优势。BOR 和 SBR 中的细菌群落多样,包括拟杆菌门、厚壁菌门、变形菌门、放线菌门和一个未鉴定的菌群。其中只有 Paracoccus sp. 被成功分离并证实具有降解 PVA 的能力。

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