一种用于提高治疗性浓缩物中凝血因子 VIII 回收率的简单血浆抗凝剂交换方法。

A simple plasma anticoagulant-exchange method to increase the recovery of factor VIII in therapeutic concentrates.

作者信息

Cumming A M, Wensley R T, Winkelman L, Lane R S

机构信息

Department of Haematology, Royal Infirmary, Manchester, UK.

出版信息

Vox Sang. 1990;58(4):264-9. doi: 10.1111/j.1423-0410.1990.tb04997.x.

DOI:10.1111/j.1423-0410.1990.tb04997.x

PMID:2119086

Abstract

Donor blood, primarily anticoagulated by acid citrate dextrose formula A (ACD-A), was separated by means of the HemaScience Autopheresis C plasmapheresis device. The citrated plasma was collected directly into a solution of heparin and calcium chloride to achieve a final plasma-ionised calcium concentration of approximately 2 mM, and a heparin concentration of 1.0 IU/ml. Heparin at this concentration provided adequate anticoagulation, and did not result in insoluble cryoprecipitates. Three pairs of donor-matched 4-kg plasma pools (anticoagulant-exchanged variant and ACD-A-anticoagulated control) were constructed and subsequently fractionated to an intermediate stage. The mean recovery of factor VIII from 3 anticoagulant-exchanged pools (394 IU/kg) was 23% greater than the mean recovery from the matched control pools (319 IU/kg). This increased recovery was not achieved at the expense of specific activity.

摘要

供体血液主要用酸性枸橼酸盐葡萄糖配方A（ACD-A）抗凝，通过HemaScience Autopheresis C血浆分离装置进行分离。将枸橼酸化血浆直接收集到肝素和氯化钙溶液中，以使最终血浆离子钙浓度约为2 mM，肝素浓度为1.0 IU/ml。此浓度的肝素可提供足够的抗凝作用，且不会导致不溶性冷沉淀。构建了三对供体匹配的4千克血浆池（抗凝剂交换变体和ACD-A抗凝对照），随后分级至中间阶段。3个抗凝剂交换血浆池（394 IU/kg）中因子VIII的平均回收率比匹配对照血浆池（319 IU/kg）的平均回收率高23%。这种回收率的提高并非以比活性为代价实现的。