Achargui S, Tijane M, Benchemsi N
Centre régional de transfusion sanguine, Madinat Al Irfane, BP 180, 10000 Rabat, Maroc.
Transfus Clin Biol. 2011 Feb;18(1):13-9. doi: 10.1016/j.tracli.2010.10.002. Epub 2010 Dec 28.
Free fetal DNA in maternal blood offers a non invasive method for prenatal diagnosis. The aim of this study is to perform fetal RHD genotyping by conventional PCR in D negative pregnant women of Moroccan origin.
Plasma sample from 120 D negative pregnant women from 10 to 40 gestational weeks were tested by conventional PCR for the presence of exons 7 and 10 of RHD gene. The results were compared with the RhD phenotype of the newborns.
In this study, the positive and the negative predictive value of the fetal RhD status were 98.7 and 96.7%, respectively.
These results demonstrate de feasibility of fetal RHD genotyping by conventional PCR in D negative pregnant women of Moroccan origin.
孕妇血液中的游离胎儿DNA为产前诊断提供了一种非侵入性方法。本研究的目的是通过常规PCR对摩洛哥裔D阴性孕妇进行胎儿RHD基因分型。
对120名孕周为10至40周的D阴性孕妇的血浆样本进行常规PCR检测，以确定RHD基因外显子7和10的存在情况。将结果与新生儿的RhD表型进行比较。
在本研究中，胎儿RhD状态的阳性预测值和阴性预测值分别为98.7%和96.7%。
这些结果证明了通过常规PCR对摩洛哥裔D阴性孕妇进行胎儿RHD基因分型的可行性。
