Pérez de Suárez E, de la Torre M, de la Hoz R
Arch Invest Med (Mex). 1978;9 Suppl 1:229-32.
Mixed and monoxenic cultures of rectal exudates from three patients with amebiasis of colon, and of feces from three asymptomatic carriers of Entamoeba histolytica obtained after administration of a saline cathartic, and from a case of exudate of the perianal cutaneous lesion border produced by this parasite, were done. The culture media used were for: mixed culture, Boeck and Drbohlav modified culture and, for monoxenic culture, Diamond's monophasic TP-S-1 medium without vitamin mixture and using bovine serum. In order to inhibit the bacterial growth in the mixed culture, streptomycin sulphate and pencillin G were used. In order to eliminate the bacterial flora kanamicin sulphate, chloramphenicol, gentamycin and disodic carbenicillin were added. Mixed cultures of seven strains of Entamoeba histolytica were obtained: HM22:IMSS, HM23:IMSS, HM24:IMSS, HM26:IMSS, HM27:IMSS, HM28:IMSS y HM29:IMSS, and monoxenic culture with Bacteroids symbiosus of the strains HM22:IMSS and HM27:IMSS, with a previous adaptation in mixed culture. The adaptation of HM26:IMSS strain in monoxenic culture was obtained by means of direct seeding of the rectal exudate with Fusobacterium symbiosus.
对三名结肠阿米巴病患者的直肠渗出物、三名服用盐水泻药后获得的溶组织内阿米巴无症状携带者的粪便，以及该寄生虫引起的肛周皮肤病变边缘渗出物病例进行了混合培养和单菌培养。所用培养基如下：混合培养采用Boeck和Drbohlav改良培养基，单菌培养采用不含维生素混合物并使用牛血清的Diamond单相TP-S-1培养基。为抑制混合培养中的细菌生长，使用了硫酸链霉素和青霉素G。为消除细菌菌群，添加了硫酸卡那霉素、氯霉素、庆大霉素和羧苄青霉素二钠。获得了七株溶组织内阿米巴的混合培养物：HM22:IMSS、HM23:IMSS、HM24:IMSS、HM26:IMSS、HM27:IMSS、HM28:IMSS和HM29:IMSS，以及菌株HM22:IMSS和HM27:IMSS与共生拟杆菌的单菌培养物，之前在混合培养中进行了适应性培养。通过将直肠渗出物与共生梭杆菌直接接种，获得了HM26:IMSS菌株在单菌培养中的适应性。
