Wang Yaling, Chai Tongjie, Lu Guozhong, Sun Lijun, Ouyang Yi, Sun Xiaodong
College of Food Science Technology, Guangdong Ocean University, Zhanjiang, Guangdong Province, P R China.
Front Biosci (Elite Ed). 2011 Jan 1;3(1):74-80. doi: 10.2741/e221.
One hundred and forty three airborne Fusarium isolates in chicken houses belonging to seven Fusarium species were analyzed by PCR with Tri5 gene as a specific marker of mycotoxin product . The result of Tri5 gene sequence analysis indicates that the PCR amplification products were 89%-96% identical to the previously reported Tri5 genes, which were all amplified from four F. poae isolates. T-2 toxin and DON was measured by immunoaffinity column and high performance liquid chromatography in Tri5-positive F. poae isolates after being cultured at constant and alternating temperatures. The production of T-2 toxin under alternating temperatures was 14 and 53 times higher than those at constant temperature of 8 °C and 25 °C. No DON was detected under either testing temperature condition. It is concluded that T-2 toxin-producing F. poae isolates were present in poultry houses, and the concentration of T-2 toxin produced by Tri5-positive F. poae isolates was increased under alternating temperatures. The application of Tri5-PCR associated with IMC-HPLC is an effective and accurate method for rapid detection of T-2 and DON mycotoxins.
以Tri5基因作为霉菌毒素产生的特异性标记，通过PCR对来自7种镰刀菌的143株鸡舍空气传播镰刀菌分离株进行了分析。Tri5基因序列分析结果表明，PCR扩增产物与先前报道的Tri5基因有89%-96%的同源性，这些基因均从4株早熟镰刀菌分离株中扩增得到。对Tri5阳性的早熟镰刀菌分离株在恒温及变温培养后，采用免疫亲和柱和高效液相色谱法测定T-2毒素和呕吐毒素。变温条件下T-2毒素的产量分别比8℃和25℃恒温条件下高14倍和53倍。在两种测试温度条件下均未检测到呕吐毒素。得出结论，家禽舍中存在产生T-2毒素的早熟镰刀菌分离株，且Tri5阳性早熟镰刀菌分离株产生的T-2毒素浓度在变温条件下有所增加。Tri5-PCR与免疫亲和柱-高效液相色谱联用是快速检测T-2毒素和呕吐毒素的有效且准确的方法。
