Capin N R, Capin R, Zamacona G, Ortiz-Ortiz L
Arch Invest Med (Mex). 1978;9 Suppl 1:291-6.
The activation of the alternative pathway of complement by axenic cultures of E. histolytica is reported. The activation is carried out in different proportions by several axenic strains, i.e., HK9:NIH, HM1:IMSS, HM2:IMSS and HM3:IMSS; the end result consists of a destruction of the parasite as well as consumption of the serum complement. The participation of the alternative pathway was studied in the presence of EGTA and Mg++, zymosan, heat-inactivation at 50 degree C for 20 min and immunoelectrophoretic studied with anti-factor B.
据报道，溶组织内阿米巴的无菌培养物可激活补体替代途径。几种无菌菌株，即HK9:NIH、HM1:IMSS、HM2:IMSS和HM3:IMSS，以不同比例进行激活；最终结果是寄生虫被破坏以及血清补体被消耗。在存在乙二醇双四乙酸（EGTA）和镁离子（Mg++）、酵母聚糖、50℃热灭活20分钟以及用抗B因子进行免疫电泳研究的情况下，对替代途径的参与情况进行了研究。
