Dong Hua, Wang Shihua, Galligan James J, Swain Greg M
Department of Entomology and Cancer Research Center, University of California, Davis, CA 95616, USA.
Front Biosci (Schol Ed). 2011 Jan 1;3(2):518-40. doi: 10.2741/s169.
Since the fabrication of the first diamond electrode in the mid 1980s, repid progress has been made on the development and application of this new type of electrode material. Boron-doped diamond (BDD) electrodes exhibit outstanding properties compared to oxygen-containing sp2 carbon electrodes. These properties make BDD electrodes an ideal choice for use in complex samples. In recent years, BDD microelectrodes have been applied to in vitro measurements of biological molecules in tissues and cells. This review will summarize recent progress in the development and applications of BDD electrodes in bio-sensing and in vitro measurements of biomolecules. In the first section, the methods for BDD diamond film deposition and BDD microelectrodes preparation are described. This is followed by a description and discussion of several approaches for characterization of the BDD electrode surface structure, morphology, and electrochemical activity. Further, application of BDD microelectrodes for use in the in vitro analysis of norepinephrine (NE), serotonin (5-HT), nitric oxide (NO), histamine, and adenosine from tissues are summarized and finally some of the remaining challenges are discussed.
自20世纪80年代中期制造出首个金刚石电极以来，这种新型电极材料的开发与应用取得了迅速进展。与含氧化合物的sp2碳电极相比，掺硼金刚石（BDD）电极具有突出的性能。这些性能使BDD电极成为用于复杂样品的理想选择。近年来，BDD微电极已应用于组织和细胞中生物分子的体外测量。本综述将总结BDD电极在生物传感和生物分子体外测量方面的开发与应用的最新进展。在第一部分，描述了BDD金刚石膜沉积和BDD微电极制备的方法。接下来是对表征BDD电极表面结构、形态和电化学活性的几种方法的描述与讨论。此外，总结了BDD微电极在组织中去甲肾上腺素（NE）、血清素（5-HT）、一氧化氮（NO）、组胺和腺苷的体外分析中的应用，最后讨论了一些尚存的挑战。
