Simons Joanne L, Vintiner Sue K
The Institute of Environmental Science and Research Ltd., Auckland, New Zealand.
J Forensic Sci. 2011 Jan;56 Suppl 1:S223-8. doi: 10.1111/j.1556-4029.2010.01595.x. Epub 2010 Oct 19.
Archived slides of cell smears treated with histological stains for sperm detection are often the only source of DNA available when cold cases are reopened. There have been conflicting reports as to the negative effects of particular histological stains on DNA recovery and quality from human cells, making stain selection an important consideration for forensic laboratories. This study investigates the effect of several staining systems on DNA recovery from histological slide samples stored from 0 to 10 weeks. DNA profiles obtained after analysis of these samples with AmpFlSTR(®) Identifiler™ and increased cycle AmpFlSTR(®) SGM Plus™ short tandem repeat (STR) profiling systems and the effects that these stains have on DNA quantity and quality over time are described. Results indicate that Christmas Tree and Hematoxylin and Eosin stains do not have significantly different effects on DNA quality after 10-week storage of slides. This research will assist scientists to select staining systems that have minimal deleterious effects on the DNA recovered.
当重新调查悬案时，经过组织学染色处理用于精子检测的细胞涂片存档玻片往往是唯一可用的DNA来源。关于特定组织学染色对从人类细胞中回收DNA及其质量的负面影响，一直存在相互矛盾的报道，这使得染色剂的选择成为法医实验室的一个重要考虑因素。本研究调查了几种染色系统对储存0至10周的组织学玻片样本中DNA回收的影响。描述了使用AmpFlSTR(®) Identifiler™和增加循环次数的AmpFlSTR(®) SGM Plus™短串联重复序列（STR）分析系统对这些样本进行分析后获得的DNA图谱，以及这些染色剂随时间推移对DNA数量和质量的影响。结果表明，在玻片储存10周后，圣诞树染色法以及苏木精和伊红染色法对DNA质量的影响没有显著差异。这项研究将帮助科学家选择对回收的DNA产生最小有害影响的染色系统。
