National Institute for Health Development, Tallinn, Estonia.
Clin Microbiol Infect. 2012 Jan;18(1):40-6. doi: 10.1111/j.1469-0691.2010.03457.x. Epub 2011 Sep 7.
Anaplasma phagocytophilum is associated with diseases of goats, sheep, cattle, dogs and horses. In the beginning of the 1990s it was identified as a human pathogen, causing human granulocytic anaplasmosis (HGA) in the USA, Europe and the far east of Russia. A. phagocytophilum is maintained in nature in an enzootic cycle including ticks as the main vector and a wide range of mammalian species as reservoirs. Ixodes ricinus and I. persulcatus ticks were collected in Estonia, Belarus and the European part of Russia and screened for the presence of A. phagocytophilum by real-time PCR. Positive samples were found only among I. ricinus, in 13.4% in the European part of Russia, 4.2% in Belarus, 1.7% in mainland Estonia and 2.6% on Saaremaa Island. Positive samples were sequenced for partial 16S rRNA, groESL and ankA genes and phylogenetic analyses were performed. The results showed that A. phagocytophilum circulating in Eastern Europe belongs to different groESL lineages and 16S rRNA gene variants and also consists of variable numbers of repetitive elements within the ankA gene.
嗜吞噬细胞无形体与山羊、绵羊、牛、狗和马的疾病有关。20 世纪 90 年代初，它被确定为人病原体，导致美国、欧洲和俄罗斯远东地区的人类粒细胞无形体病（HGA）。在自然环境中，嗜吞噬细胞无形体通过包括蜱作为主要媒介和多种哺乳动物作为宿主的地方性循环得以维持。在爱沙尼亚、白俄罗斯和俄罗斯欧洲部分采集了蓖子硬蜱和扇头蜱，并通过实时 PCR 筛选是否存在嗜吞噬细胞无形体。仅在欧洲部分的蓖子硬蜱中发现了阳性样本，俄罗斯欧洲部分为 13.4%，白俄罗斯为 4.2%，爱沙尼亚大陆为 1.7%，萨雷马岛为 2.6%。对阳性样本进行了部分 16S rRNA、groESL 和 ankA 基因的测序，并进行了系统发育分析。结果表明，在东欧循环的嗜吞噬细胞无形体属于不同的 groESL 谱系和 16S rRNA 基因变体，ankA 基因内也包含可变数量的重复元件。
