Zheng Y C
Department of Dermatology, Union Hospital, Tongji Medical University, Wuhan.
Chin Med J (Engl). 1990 Jun;103(6):489-92.
Clinical, experimental and electronmicroscopic observations, and computer-enhanced images were made on 5 patients with tinea favosa resistant to griseofulvin. The pathologic fungus was identified as the Mongolian variant of Trichophyton schoenleini. The results of physical examinations, routine tests of blood, urine and stool, liver and immunologic function examinations were normal in all of the 5 patients. Under scanning electron microscopy, the surface of the spore showed pineapple-like form, the cluster of the acrogenous spores presented flower shape, and the antler-like hyphae appeared twisted in the culture. They were observed by transmission electron microscope and the images were processed by microcomputer. It was found that the cell walls of the fungi consisted of 8 layers, among which the inner layer was loose and contained cytoplasm. It was also found that all structures within the cytoplasm possessed a 1-3 layer intact envelope and there was chromatin in the nucleus. These may be contributing factors in the development of resistance to griseofulvin. This multiple-layered thick cell wall may act as a barrier responsible for the impermeability of the cell of fungi to griseofulvin.
对5例对灰黄霉素耐药的黄癣患者进行了临床、实验及电子显微镜观察，并采用计算机增强图像技术。病理真菌被鉴定为许兰毛癣菌的蒙古变种。5例患者的体格检查、血、尿、便常规检查、肝脏及免疫功能检查结果均正常。在扫描电子显微镜下，孢子表面呈菠萝样形态，顶生孢子簇呈花形，鹿角状菌丝在培养物中呈扭曲状。通过透射电子显微镜观察并经微机处理图像。发现真菌细胞壁由8层组成，其中内层疏松并含有细胞质。还发现细胞质内所有结构都有1 - 3层完整包膜，细胞核内有染色质。这些可能是对灰黄霉素产生耐药性的因素。这种多层厚细胞壁可能作为一种屏障，导致真菌细胞对灰黄霉素不渗透。
