Cogné M, Mounir S, Aucouturier P, Preud'homme J L, Nau F, Guglielmi P
CNRS URA 1172, Faculté des Sciences, Poitiers, France.
Eur J Immunol. 1990 Sep;20(9):1905-10. doi: 10.1002/eji.1830200906.
The Burkitt lymphoma cell lines Ly91, Ly47 and Ly66 produce short mu chains in the absence of associated light (L) chains. mu mRNA lack two-thirds of the variable (V) region due to an alternate splicing event that takes place within V genes. L chain genes were found to be rearranged in these lines and to lead to transcripts with altered V regions. Although these abnormal transcripts displayed specific abnormalities in each cell line, they shared some features, including large deletions involving the V-J junction. In one cell line, L chain transcripts made up of either V kappa I subgroup gene J kappa 3 or V kappa IV gene-J kappa 4 recombination products bore a similar alteration: the donor splice site of the rearranged J kappa segment was modified, leading to the alternate use of the donor site of either the leader peptide exon or the next 3' unrearranged J kappa segment.
伯基特淋巴瘤细胞系Ly91、Ly47和Ly66在缺乏相关轻链（L链）的情况下产生短μ链。由于V基因内发生的选择性剪接事件，μ mRNA缺乏三分之二的可变（V）区。发现L链基因在这些细胞系中发生重排，并导致V区改变的转录本。尽管这些异常转录本在每个细胞系中表现出特定的异常，但它们具有一些共同特征，包括涉及V-J连接的大片段缺失。在一个细胞系中，由VκI亚组基因Jκ3或VκIV基因-Jκ4重组产物组成的L链转录本有类似改变：重排的Jκ片段的供体剪接位点被修饰，导致前导肽外显子或下一个3'未重排的Jκ片段的供体位点被选择性使用。
