Beaver R W, Wilson D M, Trucksess M W
University of Georgia, Mycotoxin Analysis Research Center, Tifton, GA 31793-0748.
J Assoc Off Anal Chem. 1990 Jul-Aug;73(4):579-81.
Quantitation of aflatoxins by liquid chromatography with postcolumn iodine derivatization (LC-PCD) and fluorescence detection was compared with quantitation by the AOAC CB method, 968.22. Thirty-seven naturally contaminated corn samples were ground and then divided. One portion was extracted, and the extract was cleaned up and analyzed by thin-layer chromatography according to the CB method. The second portion was extracted and cleaned up in a similar fashion, but quantitation was by the LC-PCD method. For aflatoxin B1 concentrations ranging from 0 to 150 ng/g, results obtained by the 2 methods were fitted to a linear equation with the LC-PCD results as the dependent variable. The correlation coefficient was 0.99, the intercept was near 0, and the slope was near 1. For aflatoxin B2, the correlation coefficient was 0.97, and the intercept was near 0. However, the slope of the equation relating LC-PCD concentration to TLC concentration was only 0.5. We believe that this lack of equivalence between the methods for determination of aflatoxin B2 is due to overestimation by the TLC method because the low levels present are near the TLC detection limit for B2.
采用柱后碘衍生化液相色谱法（LC - PCD）和荧光检测法定量黄曲霉毒素，并与AOAC的CB方法968.22进行比较。将37个天然污染的玉米样品研磨后进行分割。一部分进行提取，提取物经净化后按照CB方法用薄层色谱法分析。第二部分以类似方式提取和净化，但采用LC - PCD法定量。对于黄曲霉毒素B1浓度范围为0至150 ng/g的情况，将两种方法得到的结果以LC - PCD结果为因变量拟合到线性方程中。相关系数为0.99，截距接近0，斜率接近1。对于黄曲霉毒素B2，相关系数为0.97，截距接近0。然而，将LC - PCD浓度与薄层色谱法浓度相关联的方程斜率仅为0.5。我们认为，两种方法测定黄曲霉毒素B2时缺乏等效性是由于薄层色谱法高估了结果，因为低含量水平接近B2的薄层色谱检测限。
