Castellano B, González B, Finsen B R, Zimmer J
Department of Cell Biology and Physiology, Autonomous University of Barcelona, Spain.
J Histochem Cytochem. 1990 Nov;38(11):1535-9. doi: 10.1177/38.11.2120328.
The histochemical localization of enzymes associated with purine nucleoside metabolism indicates that glial cells might participate in the regulation of these compounds in the central nervous system. In the present study we examined the histochemical localization of purine nucleoside phosphorylase (PNPase) in sections from adult rat brain. Some sections were also sequentially stained immunocytochemically for astroglial or microglial cells utilizing glial fibrillary acidic protein (GFAP) or OX-42 antibodies, respectively. Our observations showed that PNPase was restricted to glial cells, whereas neurons always remained negative. Brain sections stained for both PNPase and GFAP showed that the GFAP-positive astroglial cells were always PNPase positive. Other PNPase-positive but GFAP-negative cells were also observed. These cells resembled microglial cells, and brain sections reacted for both PNPase and OX-42 confirmed this by showing that the major part of OX-42-positive microglial cells were PNPase positive. In these sections, the PNPase-positive but OX-42-negative cells present resembled astroglial cells. From our double staining experiments, we conclude that PNPase is present in both astroglial and microglial cells in normal adult brain.
与嘌呤核苷代谢相关的酶的组织化学定位表明，神经胶质细胞可能参与中枢神经系统中这些化合物的调节。在本研究中，我们检查了成年大鼠脑切片中嘌呤核苷磷酸化酶（PNPase）的组织化学定位。一些切片还分别利用胶质纤维酸性蛋白（GFAP）或OX - 42抗体对星形胶质细胞或小胶质细胞进行了免疫细胞化学连续染色。我们的观察结果表明，PNPase仅限于神经胶质细胞，而神经元始终呈阴性。对PNPase和GFAP进行染色的脑切片显示，GFAP阳性的星形胶质细胞总是PNPase阳性。还观察到其他PNPase阳性但GFAP阴性的细胞。这些细胞类似于小胶质细胞，对PNPase和OX - 42进行反应的脑切片通过显示OX - 42阳性小胶质细胞的主要部分为PNPase阳性证实了这一点。在这些切片中，存在的PNPase阳性但OX - 42阴性的细胞类似于星形胶质细胞。从我们的双重染色实验中，我们得出结论，在正常成年大脑中，PNPase存在于星形胶质细胞和小胶质细胞中。
