Wood C R, Dorner A J, Morris G E, Alderman E M, Wilson D, O'Hara R M, Kaufman R J
Genetics Institute, Inc., Cambridge, MA 02140.
J Immunol. 1990 Nov 1;145(9):3011-6.
The expression of lambda L and microH chain cDNA was examined in Chinese hamster ovary cells. Each cDNA was linked to a different, amplifiable, selectable drug marker gene, and expression was monitored in the presence of increasing concentrations of the selective drugs. Cells were obtained that produced greater than 60 micrograms/10(6) cells/48 h of assembled antibody. This Chinese hamster ovary cell-synthesized IgM was polymeric, and exhibited specific hapten binding and C fixation. The expression strategy employed here may prove useful for the future production of genetically engineered antibodies and other multi-subunit proteins.
在中国仓鼠卵巢细胞中检测了λ轻链和μ重链cDNA的表达。每个cDNA都与一个不同的、可扩增的、可选择的药物标记基因相连，并在存在递增浓度的选择药物的情况下监测表达情况。获得了每48小时每10⁶个细胞产生超过60微克组装抗体的细胞。这种中国仓鼠卵巢细胞合成的IgM是多聚体的，并表现出特异性半抗原结合和补体固定。这里采用的表达策略可能对未来生产基因工程抗体和其他多亚基蛋白有用。
