Department of Medical Laboratory Sciences, Faculty of Health Sciences, Hokkaido University, Nishi-5 Kita-12, Kitaku, Sapporo, Hokkaido, 060-0812, Japan.
J Infect Chemother. 2011 Aug;17(4):478-82. doi: 10.1007/s10156-010-0199-5. Epub 2011 Jan 5.
The survival of Alloiococcus otitidis (NCFB2890) with different nutritional supplements, including brain-heart infusion broth (BHI), phosphate-buffered saline (PBS), distilled water (DW), and middle ear effusion (MEE), as well as various atmospheres (aerobic, microaerobic, anaerobic), was compared using cultures, LIVE/DEAD staining, and transmission electron microscopy. The bacterial morphological traits and viability were maintained in BHI and MEE under aerobic conditions but were rapidly lost in PBS and DW. In contrast, anaerobic conditions did not support viability at all. Thus, the bacteria critically required an aerobic atmosphere for its survival as well as the appropriate nutrients, implying that culture of this pathogen from clinical specimens would become more difficult through oxygen depletion depending on a slight change in the middle ear atmosphere.
采用培养、LIVE/DEAD 染色和透射电子显微镜技术，比较了不同营养补充物（包括脑心浸液肉汤（BHI）、磷酸盐缓冲盐水（PBS）、蒸馏水（DW）和中耳积液（MEE））以及不同大气条件（需氧、微需氧、厌氧）下，黏性奥尔森菌（NCFB2890）的存活情况。在需氧条件下，BHI 和 MEE 中细菌的形态特征和活力得以维持，但在 PBS 和 DW 中则迅速丧失。相反，厌氧条件根本不支持活力。因此，细菌的存活需要有氧气氛和适当的营养物质，这意味着这种病原体从临床标本中的培养将变得更加困难，因为氧的消耗会导致中耳气氛的微小变化。
