Department of Biochemistry and Molecular Biology, The University of Texas Medical School at Houston, Houston, TX 77030, USA.
Protein Cell. 2010 Mar;1(3):227-36. doi: 10.1007/s13238-010-0032-z. Epub 2010 Feb 23.
Eukaryotic DNA replication is tightly restricted to only once per cell cycle in order to maintain genome stability. Cells use multiple mechanisms to control the assembly of the prereplication complex (pre-RC), a process known as replication licensing. This review focuses on the regulation of replication licensing by posttranslational modifications of the licensing factors, including phosphorylation, ubiquitylation and acetylation. These modifications are critical in establishing the pre-RC complexes as well as preventing rereplication in each cell cycle. The relationship between rereplication and diseases, including cancer and virus infection, is discussed as well.
真核生物的 DNA 复制在细胞周期中严格限制仅进行一次，以维持基因组稳定性。细胞利用多种机制来控制前复制复合物（pre-RC）的组装，这个过程被称为复制许可。本综述重点介绍了通过许可因子的翻译后修饰（包括磷酸化、泛素化和乙酰化）对复制许可的调控。这些修饰对于建立 pre-RC 复合物以及防止每个细胞周期中的重复复制至关重要。还讨论了重复复制与疾病（包括癌症和病毒感染）之间的关系。
